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The septin cytoskeleton facilitates membrane retraction during motility and blebbing

Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the plasma membrane, focusing on this cytoskeletal...

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Autores principales: Gilden, Julia K., Peck, Sebastian, M. Chen, Yi-Chun, Krummel, Matthew F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255977/
https://www.ncbi.nlm.nih.gov/pubmed/22232702
http://dx.doi.org/10.1083/jcb.201105127
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author Gilden, Julia K.
Peck, Sebastian
M. Chen, Yi-Chun
Krummel, Matthew F.
author_facet Gilden, Julia K.
Peck, Sebastian
M. Chen, Yi-Chun
Krummel, Matthew F.
author_sort Gilden, Julia K.
collection PubMed
description Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the plasma membrane, focusing on this cytoskeletal element’s role during effective amoeboid motility. Surprisingly, septins play a reactive rather than proactive role, as demonstrated during the response to increasing hydrostatic pressure and subsequent regulatory volume decrease. In these settings, septins were required for rapid cortical contraction, and SEPT6-GFP was recruited into filaments and circular patches during global cortical contraction and also specifically during actin filament depletion. Recruitment of septins was also evident during excessive blebbing initiated by blocking membrane trafficking with a dynamin inhibitor, providing further evidence that septins are recruited to facilitate retraction of membranes during dynamic shape change. This function of septins in assembling on an unstable cortex and retracting aberrantly protruding membranes explains the excessive blebbing and protrusion observed in septin-deficient T cells.
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spelling pubmed-32559772012-07-09 The septin cytoskeleton facilitates membrane retraction during motility and blebbing Gilden, Julia K. Peck, Sebastian M. Chen, Yi-Chun Krummel, Matthew F. J Cell Biol Research Articles Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the plasma membrane, focusing on this cytoskeletal element’s role during effective amoeboid motility. Surprisingly, septins play a reactive rather than proactive role, as demonstrated during the response to increasing hydrostatic pressure and subsequent regulatory volume decrease. In these settings, septins were required for rapid cortical contraction, and SEPT6-GFP was recruited into filaments and circular patches during global cortical contraction and also specifically during actin filament depletion. Recruitment of septins was also evident during excessive blebbing initiated by blocking membrane trafficking with a dynamin inhibitor, providing further evidence that septins are recruited to facilitate retraction of membranes during dynamic shape change. This function of septins in assembling on an unstable cortex and retracting aberrantly protruding membranes explains the excessive blebbing and protrusion observed in septin-deficient T cells. The Rockefeller University Press 2012-01-09 /pmc/articles/PMC3255977/ /pubmed/22232702 http://dx.doi.org/10.1083/jcb.201105127 Text en © 2012 Gilden et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Gilden, Julia K.
Peck, Sebastian
M. Chen, Yi-Chun
Krummel, Matthew F.
The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title_full The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title_fullStr The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title_full_unstemmed The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title_short The septin cytoskeleton facilitates membrane retraction during motility and blebbing
title_sort septin cytoskeleton facilitates membrane retraction during motility and blebbing
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255977/
https://www.ncbi.nlm.nih.gov/pubmed/22232702
http://dx.doi.org/10.1083/jcb.201105127
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