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dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions

Notch signaling governs binary cell fate determination in asymmetrically dividing cells. Through a forward genetic screen we identified the fly homologue of Eps15 homology domain containing protein-binding protein 1 (dEHBP1) as a novel regulator of Notch signaling in asymmetrically dividing cells. d...

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Autores principales: Giagtzoglou, Nikolaos, Yamamoto, Shinya, Zitserman, Diana, Graves, Hillary K., Schulze, Karen L., Wang, Hao, Klein, Hayley, Roegiers, Fabrice, Bellen, Hugo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255984/
https://www.ncbi.nlm.nih.gov/pubmed/22213802
http://dx.doi.org/10.1083/jcb.201106088
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author Giagtzoglou, Nikolaos
Yamamoto, Shinya
Zitserman, Diana
Graves, Hillary K.
Schulze, Karen L.
Wang, Hao
Klein, Hayley
Roegiers, Fabrice
Bellen, Hugo J.
author_facet Giagtzoglou, Nikolaos
Yamamoto, Shinya
Zitserman, Diana
Graves, Hillary K.
Schulze, Karen L.
Wang, Hao
Klein, Hayley
Roegiers, Fabrice
Bellen, Hugo J.
author_sort Giagtzoglou, Nikolaos
collection PubMed
description Notch signaling governs binary cell fate determination in asymmetrically dividing cells. Through a forward genetic screen we identified the fly homologue of Eps15 homology domain containing protein-binding protein 1 (dEHBP1) as a novel regulator of Notch signaling in asymmetrically dividing cells. dEHBP1 is enriched basally and at the actin-rich interface of pII cells of the external mechanosensory organs, where Notch signaling occurs. Loss of function of dEHBP1 leads to up-regulation of Sanpodo, a regulator of Notch signaling, and aberrant trafficking of the Notch ligand, Delta. Furthermore, Sec15 and Rab11, which have been previously shown to regulate the localization of Delta, physically interact with dEHBP1. We propose that dEHBP1 functions as an adaptor molecule for the exocytosis and recycling of Delta, thereby affecting cell fate decisions in asymmetrically dividing cells.
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spelling pubmed-32559842012-07-09 dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions Giagtzoglou, Nikolaos Yamamoto, Shinya Zitserman, Diana Graves, Hillary K. Schulze, Karen L. Wang, Hao Klein, Hayley Roegiers, Fabrice Bellen, Hugo J. J Cell Biol Research Articles Notch signaling governs binary cell fate determination in asymmetrically dividing cells. Through a forward genetic screen we identified the fly homologue of Eps15 homology domain containing protein-binding protein 1 (dEHBP1) as a novel regulator of Notch signaling in asymmetrically dividing cells. dEHBP1 is enriched basally and at the actin-rich interface of pII cells of the external mechanosensory organs, where Notch signaling occurs. Loss of function of dEHBP1 leads to up-regulation of Sanpodo, a regulator of Notch signaling, and aberrant trafficking of the Notch ligand, Delta. Furthermore, Sec15 and Rab11, which have been previously shown to regulate the localization of Delta, physically interact with dEHBP1. We propose that dEHBP1 functions as an adaptor molecule for the exocytosis and recycling of Delta, thereby affecting cell fate decisions in asymmetrically dividing cells. The Rockefeller University Press 2012-01-09 /pmc/articles/PMC3255984/ /pubmed/22213802 http://dx.doi.org/10.1083/jcb.201106088 Text en © 2012 Giagtzoglou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Giagtzoglou, Nikolaos
Yamamoto, Shinya
Zitserman, Diana
Graves, Hillary K.
Schulze, Karen L.
Wang, Hao
Klein, Hayley
Roegiers, Fabrice
Bellen, Hugo J.
dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title_full dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title_fullStr dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title_full_unstemmed dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title_short dEHBP1 controls exocytosis and recycling of Delta during asymmetric divisions
title_sort dehbp1 controls exocytosis and recycling of delta during asymmetric divisions
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255984/
https://www.ncbi.nlm.nih.gov/pubmed/22213802
http://dx.doi.org/10.1083/jcb.201106088
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