Cargando…
Amplification by PCR Artificially Reduces the Proportion of the Rare Biosphere in Microbial Communities
The microbial world has been shown to hold an unimaginable diversity. The use of rRNA genes and PCR amplification to assess microbial community structure and diversity present biases that need to be analyzed in order to understand the risks involved in those estimates. Herein, we show that PCR ampli...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256211/ https://www.ncbi.nlm.nih.gov/pubmed/22253843 http://dx.doi.org/10.1371/journal.pone.0029973 |