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Two-Stage Clustering (TSC): A Pipeline for Selecting Operational Taxonomic Units for the High-Throughput Sequencing of PCR Amplicons

Clustering 16S/18S rRNA amplicon sequences into operational taxonomic units (OTUs) is a critical step for the bioinformatic analysis of microbial diversity. Here, we report a pipeline for selecting OTUs with a relatively low computational demand and a high degree of accuracy. This pipeline is referr...

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Detalles Bibliográficos
Autores principales:	Jiang, Xiao-Tao, Zhang, Hai, Sheng, Hua-Fang, Wang, Yu, He, Yan, Zou, Fei, Zhou, Hong-Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256218/ https://www.ncbi.nlm.nih.gov/pubmed/22253923 http://dx.doi.org/10.1371/journal.pone.0030230

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