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Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation

Histone deacetylase inhibitors (HDACi) represent a promising avenue for cancer therapy. We applied mass spectrometry (MS) to determine the impact of clinically relevant HDACi on global levels of histone acetylation. Intact histone profiling revealed that the HDACi SAHA and MS-275 globally increased...

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Autores principales: Drogaris, Paul, Villeneuve, Valérie, Pomiès, Christelle, Lee, Eun-Hye, Bourdeau, Véronique, Bonneil, Éric, Ferbeyre, Gerardo, Verreault, Alain, Thibault, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256565/
https://www.ncbi.nlm.nih.gov/pubmed/22355734
http://dx.doi.org/10.1038/srep00220
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author Drogaris, Paul
Villeneuve, Valérie
Pomiès, Christelle
Lee, Eun-Hye
Bourdeau, Véronique
Bonneil, Éric
Ferbeyre, Gerardo
Verreault, Alain
Thibault, Pierre
author_facet Drogaris, Paul
Villeneuve, Valérie
Pomiès, Christelle
Lee, Eun-Hye
Bourdeau, Véronique
Bonneil, Éric
Ferbeyre, Gerardo
Verreault, Alain
Thibault, Pierre
author_sort Drogaris, Paul
collection PubMed
description Histone deacetylase inhibitors (HDACi) represent a promising avenue for cancer therapy. We applied mass spectrometry (MS) to determine the impact of clinically relevant HDACi on global levels of histone acetylation. Intact histone profiling revealed that the HDACi SAHA and MS-275 globally increased histone H3 and H4 acetylation in both normal diploid fibroblasts and transformed human cells. Histone H3 lysine 56 acetylation (H3K56ac) recently elicited much interest and controversy due to its potential as a diagnostic and prognostic marker for a broad diversity of cancers. Using quantitative MS, we demonstrate that H3K56ac is much less abundant than previously reported in human cells. Unexpectedly, in contrast to H3/H4 N-terminal tail acetylation, H3K56ac did not increase in response to inhibitors of each class of HDACs. In addition, we demonstrate that antibodies raised against H3K56ac peptides cross-react against H3 N-terminal tail acetylation sites that carry sequence similarity to residues flanking H3K56.
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spelling pubmed-32565652012-01-12 Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation Drogaris, Paul Villeneuve, Valérie Pomiès, Christelle Lee, Eun-Hye Bourdeau, Véronique Bonneil, Éric Ferbeyre, Gerardo Verreault, Alain Thibault, Pierre Sci Rep Article Histone deacetylase inhibitors (HDACi) represent a promising avenue for cancer therapy. We applied mass spectrometry (MS) to determine the impact of clinically relevant HDACi on global levels of histone acetylation. Intact histone profiling revealed that the HDACi SAHA and MS-275 globally increased histone H3 and H4 acetylation in both normal diploid fibroblasts and transformed human cells. Histone H3 lysine 56 acetylation (H3K56ac) recently elicited much interest and controversy due to its potential as a diagnostic and prognostic marker for a broad diversity of cancers. Using quantitative MS, we demonstrate that H3K56ac is much less abundant than previously reported in human cells. Unexpectedly, in contrast to H3/H4 N-terminal tail acetylation, H3K56ac did not increase in response to inhibitors of each class of HDACs. In addition, we demonstrate that antibodies raised against H3K56ac peptides cross-react against H3 N-terminal tail acetylation sites that carry sequence similarity to residues flanking H3K56. Nature Publishing Group 2012-01-12 /pmc/articles/PMC3256565/ /pubmed/22355734 http://dx.doi.org/10.1038/srep00220 Text en Copyright © 2012, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Drogaris, Paul
Villeneuve, Valérie
Pomiès, Christelle
Lee, Eun-Hye
Bourdeau, Véronique
Bonneil, Éric
Ferbeyre, Gerardo
Verreault, Alain
Thibault, Pierre
Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title_full Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title_fullStr Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title_full_unstemmed Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title_short Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
title_sort histone deacetylase inhibitors globally enhance h3/h4 tail acetylation without affecting h3 lysine 56 acetylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256565/
https://www.ncbi.nlm.nih.gov/pubmed/22355734
http://dx.doi.org/10.1038/srep00220
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