Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing

Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-...

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Detalles Bibliográficos
Autores principales: Jin, Hongjun, Yu, Yuehua, Chrisler, William B., Xiong, Yijia, Hu, Dehong, Lei, Chenghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2011
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256732/
https://www.ncbi.nlm.nih.gov/pubmed/22259248
http://dx.doi.org/10.4137/BCBCR.S8513
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author Jin, Hongjun
Yu, Yuehua
Chrisler, William B.
Xiong, Yijia
Hu, Dehong
Lei, Chenghong
author_facet Jin, Hongjun
Yu, Yuehua
Chrisler, William B.
Xiong, Yijia
Hu, Dehong
Lei, Chenghong
author_sort Jin, Hongjun
collection PubMed
description Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.
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spelling pubmed-32567322012-01-18 Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing Jin, Hongjun Yu, Yuehua Chrisler, William B. Xiong, Yijia Hu, Dehong Lei, Chenghong Breast Cancer (Auckl) Original Research Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness. Libertas Academica 2011-12-07 /pmc/articles/PMC3256732/ /pubmed/22259248 http://dx.doi.org/10.4137/BCBCR.S8513 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Jin, Hongjun
Yu, Yuehua
Chrisler, William B.
Xiong, Yijia
Hu, Dehong
Lei, Chenghong
Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title_full Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title_fullStr Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title_full_unstemmed Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title_short Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
title_sort delivery of microrna-10b with polylysine nanoparticles for inhibition of breast cancer cell wound healing
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256732/
https://www.ncbi.nlm.nih.gov/pubmed/22259248
http://dx.doi.org/10.4137/BCBCR.S8513
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