Cargando…

Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1

To further investigate the potential role of α-tocopherol in maintaining immuno-homeostasis in bovine cells (Madin-Darby bovine kidney epithelial cell line), we undertook in vitro experiments using recombinant TNF-α as an immuno-stimulant to simulate inflammation response in cells with or without α-...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Cong-Jun, Li, Robert W., Kahl, Stanislaw, Elsasser, Theodore H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256998/
https://www.ncbi.nlm.nih.gov/pubmed/22267916
http://dx.doi.org/10.4137/GRSB.S8303
_version_ 1782221088836550656
author Li, Cong-Jun
Li, Robert W.
Kahl, Stanislaw
Elsasser, Theodore H.
author_facet Li, Cong-Jun
Li, Robert W.
Kahl, Stanislaw
Elsasser, Theodore H.
author_sort Li, Cong-Jun
collection PubMed
description To further investigate the potential role of α-tocopherol in maintaining immuno-homeostasis in bovine cells (Madin-Darby bovine kidney epithelial cell line), we undertook in vitro experiments using recombinant TNF-α as an immuno-stimulant to simulate inflammation response in cells with or without α-tocopherol pre-treatment. Using microarray global-profiling and IPA (Ingenuity Pathways Analysis, Ingenuity(®) Systems, http://www.ingenuity.com) data analysis on TNF-α-induced gene perturbation in those cells, we focused on determining whether α-tocopherol treatment of normal bovine cells in a standard cell culture condition can modify cell’s immune response induced by TNF-α challenge. When three datasets were filtered and compared using IPA, there were a total of 1750 genes in all three datasets for comparison, 97 genes were common in all three sets; 615 genes were common in at least two datasets; there were 261 genes unique in TNF-α challenge, 399 genes were unique in α-tocopherol treatment, and 378 genes were unique in the α-tocopherol plus TNF-α treatment. TNF-α challenge induced significant change in gene expression. Many of those genes induced by TNF-α are related to the cells immune and inflammatory responses. The results of IPA data analysis showed that α-tocopherol-pretreatment of cells modulated cell’s response to TNF-α challenge. In most of the canonical pathways, α-tocopherol pretreatment showed the antagonistic effect against the TNF-α-induced pro-inflammatory responses. We concluded that α-tocopherol pre-treatment has a significant antagonistic effect that modulates the cell’s response to the TNF-α challenge by altering the gene expression activities of some important signaling molecules.
format Online
Article
Text
id pubmed-3256998
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Libertas Academica
record_format MEDLINE/PubMed
spelling pubmed-32569982012-01-20 Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1 Li, Cong-Jun Li, Robert W. Kahl, Stanislaw Elsasser, Theodore H. Gene Regul Syst Bio Original Research To further investigate the potential role of α-tocopherol in maintaining immuno-homeostasis in bovine cells (Madin-Darby bovine kidney epithelial cell line), we undertook in vitro experiments using recombinant TNF-α as an immuno-stimulant to simulate inflammation response in cells with or without α-tocopherol pre-treatment. Using microarray global-profiling and IPA (Ingenuity Pathways Analysis, Ingenuity(®) Systems, http://www.ingenuity.com) data analysis on TNF-α-induced gene perturbation in those cells, we focused on determining whether α-tocopherol treatment of normal bovine cells in a standard cell culture condition can modify cell’s immune response induced by TNF-α challenge. When three datasets were filtered and compared using IPA, there were a total of 1750 genes in all three datasets for comparison, 97 genes were common in all three sets; 615 genes were common in at least two datasets; there were 261 genes unique in TNF-α challenge, 399 genes were unique in α-tocopherol treatment, and 378 genes were unique in the α-tocopherol plus TNF-α treatment. TNF-α challenge induced significant change in gene expression. Many of those genes induced by TNF-α are related to the cells immune and inflammatory responses. The results of IPA data analysis showed that α-tocopherol-pretreatment of cells modulated cell’s response to TNF-α challenge. In most of the canonical pathways, α-tocopherol pretreatment showed the antagonistic effect against the TNF-α-induced pro-inflammatory responses. We concluded that α-tocopherol pre-treatment has a significant antagonistic effect that modulates the cell’s response to the TNF-α challenge by altering the gene expression activities of some important signaling molecules. Libertas Academica 2011-12-05 /pmc/articles/PMC3256998/ /pubmed/22267916 http://dx.doi.org/10.4137/GRSB.S8303 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Original Research
Li, Cong-Jun
Li, Robert W.
Kahl, Stanislaw
Elsasser, Theodore H.
Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title_full Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title_fullStr Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title_full_unstemmed Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title_short Alpha-Tocopherol Alters Transcription Activities that Modulates Tumor Necrosis Factor Alpha (TNF-α) Induced Inflammatory Response in Bovine Cells1
title_sort alpha-tocopherol alters transcription activities that modulates tumor necrosis factor alpha (tnf-α) induced inflammatory response in bovine cells1
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256998/
https://www.ncbi.nlm.nih.gov/pubmed/22267916
http://dx.doi.org/10.4137/GRSB.S8303
work_keys_str_mv AT licongjun alphatocopherolalterstranscriptionactivitiesthatmodulatestumornecrosisfactoralphatnfainducedinflammatoryresponseinbovinecells1
AT lirobertw alphatocopherolalterstranscriptionactivitiesthatmodulatestumornecrosisfactoralphatnfainducedinflammatoryresponseinbovinecells1
AT kahlstanislaw alphatocopherolalterstranscriptionactivitiesthatmodulatestumornecrosisfactoralphatnfainducedinflammatoryresponseinbovinecells1
AT elsassertheodoreh alphatocopherolalterstranscriptionactivitiesthatmodulatestumornecrosisfactoralphatnfainducedinflammatoryresponseinbovinecells1