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Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds
A major hurdle in studying biological systems and administering effective tissue engineered therapies is the lack of suitable cell culture models that replicate the dynamic nature of cell-microenvironment interactions. Advances in the field of surface chemistry and polymer science have allowed resea...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257091/ https://www.ncbi.nlm.nih.gov/pubmed/22272094 http://dx.doi.org/10.3390/ijms12128596 |
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author | Bajpai, Saumendra Kim, Na Young Reinhart-King, Cynthia A. |
author_facet | Bajpai, Saumendra Kim, Na Young Reinhart-King, Cynthia A. |
author_sort | Bajpai, Saumendra |
collection | PubMed |
description | A major hurdle in studying biological systems and administering effective tissue engineered therapies is the lack of suitable cell culture models that replicate the dynamic nature of cell-microenvironment interactions. Advances in the field of surface chemistry and polymer science have allowed researchers to develop novel methodologies to manipulate materials to be extrinsically tunable. Usage of such materials in modeling tissues in vitro has offered valuable insights into numerous cellular processes including motility, invasion, and alterations in cell morphology. Here, we discuss novel techniques devised to more closely mimic cell-tissue interactions and to study cell response to distinct physico-chemical changes in biomaterials, with an emphasis on the manipulation of collagen scaffolds. The benefits and pitfalls associated with using collagen are discussed in the context of strategies proposed to control the engineered microenvironment. Tunable systems such as these offer the ability to alter individual features of the microenvironment in vitro, with the promise that the molecular basis of mechanotransduction in vivo may be laid out in future. |
format | Online Article Text |
id | pubmed-3257091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32570912012-01-23 Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds Bajpai, Saumendra Kim, Na Young Reinhart-King, Cynthia A. Int J Mol Sci Review A major hurdle in studying biological systems and administering effective tissue engineered therapies is the lack of suitable cell culture models that replicate the dynamic nature of cell-microenvironment interactions. Advances in the field of surface chemistry and polymer science have allowed researchers to develop novel methodologies to manipulate materials to be extrinsically tunable. Usage of such materials in modeling tissues in vitro has offered valuable insights into numerous cellular processes including motility, invasion, and alterations in cell morphology. Here, we discuss novel techniques devised to more closely mimic cell-tissue interactions and to study cell response to distinct physico-chemical changes in biomaterials, with an emphasis on the manipulation of collagen scaffolds. The benefits and pitfalls associated with using collagen are discussed in the context of strategies proposed to control the engineered microenvironment. Tunable systems such as these offer the ability to alter individual features of the microenvironment in vitro, with the promise that the molecular basis of mechanotransduction in vivo may be laid out in future. Molecular Diversity Preservation International (MDPI) 2011-11-29 /pmc/articles/PMC3257091/ /pubmed/22272094 http://dx.doi.org/10.3390/ijms12128596 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Bajpai, Saumendra Kim, Na Young Reinhart-King, Cynthia A. Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title | Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title_full | Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title_fullStr | Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title_full_unstemmed | Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title_short | Approaches to Manipulating the Dimensionality and Physicochemical Properties of Common Cellular Scaffolds |
title_sort | approaches to manipulating the dimensionality and physicochemical properties of common cellular scaffolds |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257091/ https://www.ncbi.nlm.nih.gov/pubmed/22272094 http://dx.doi.org/10.3390/ijms12128596 |
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