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Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate
The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were ident...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257242/ https://www.ncbi.nlm.nih.gov/pubmed/22253719 http://dx.doi.org/10.1371/journal.pone.0029392 |
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author | Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun |
author_facet | Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun |
author_sort | Li, Robert W. |
collection | PubMed |
description | The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were identified in the rumen microbiota of dairy cows. The rumen microbiota harbored 54.5±6.1 genera (mean ± SD) and 127.3±4.4 operational taxonomic units (OTUs), respectively. However, the core microbiome comprised of 26 genera and 82 OTUs. Butyrate infusion altered molar percentages of 3 major VFAs. Butyrate perturbation had a profound impact on the rumen microbial composition. A 72 h-infusion led to a significant change in the numbers of sequence reads derived from 4 phyla, including 2 most abundant phyla, Bacteroidetes and Firmicutes. As many as 19 genera and 43 OTUs were significantly impacted by butyrate infusion. Elevated butyrate levels in the rumen seemingly had a stimulating effect on butyrate-producing bacteria populations. The resilience of the rumen microbial ecosystem was evident as the abundance of the microorganisms returned to their pre-disturbed status after infusion withdrawal. Our findings provide insight into perturbation dynamics of the rumen microbial ecosystem and should guide efforts in formulating optimal uses of probiotic bacteria treating human diseases. |
format | Online Article Text |
id | pubmed-3257242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32572422012-01-17 Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun PLoS One Research Article The capacity of the rumen microbiota to produce volatile fatty acids (VFAs) has important implications in animal well-being and production. We investigated temporal changes of the rumen microbiota in response to butyrate infusion using pyrosequencing of the 16S rRNA gene. Twenty one phyla were identified in the rumen microbiota of dairy cows. The rumen microbiota harbored 54.5±6.1 genera (mean ± SD) and 127.3±4.4 operational taxonomic units (OTUs), respectively. However, the core microbiome comprised of 26 genera and 82 OTUs. Butyrate infusion altered molar percentages of 3 major VFAs. Butyrate perturbation had a profound impact on the rumen microbial composition. A 72 h-infusion led to a significant change in the numbers of sequence reads derived from 4 phyla, including 2 most abundant phyla, Bacteroidetes and Firmicutes. As many as 19 genera and 43 OTUs were significantly impacted by butyrate infusion. Elevated butyrate levels in the rumen seemingly had a stimulating effect on butyrate-producing bacteria populations. The resilience of the rumen microbial ecosystem was evident as the abundance of the microorganisms returned to their pre-disturbed status after infusion withdrawal. Our findings provide insight into perturbation dynamics of the rumen microbial ecosystem and should guide efforts in formulating optimal uses of probiotic bacteria treating human diseases. Public Library of Science 2012-01-12 /pmc/articles/PMC3257242/ /pubmed/22253719 http://dx.doi.org/10.1371/journal.pone.0029392 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Li, Robert W. Wu, Sitao Baldwin, Ransom L. Li, Weizhong Li, Congjun Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title | Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title_full | Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title_fullStr | Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title_full_unstemmed | Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title_short | Perturbation Dynamics of the Rumen Microbiota in Response to Exogenous Butyrate |
title_sort | perturbation dynamics of the rumen microbiota in response to exogenous butyrate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257242/ https://www.ncbi.nlm.nih.gov/pubmed/22253719 http://dx.doi.org/10.1371/journal.pone.0029392 |
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