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Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin

Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrin-independent endocytic pathways have been described in o...

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Autores principales: Prosser, Derek C., Drivas, Theodore G., Maldonado-Báez, Lymarie, Wendland, Beverly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257529/
https://www.ncbi.nlm.nih.gov/pubmed/22065638
http://dx.doi.org/10.1083/jcb.201104045
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author Prosser, Derek C.
Drivas, Theodore G.
Maldonado-Báez, Lymarie
Wendland, Beverly
author_facet Prosser, Derek C.
Drivas, Theodore G.
Maldonado-Báez, Lymarie
Wendland, Beverly
author_sort Prosser, Derek C.
collection PubMed
description Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrin-independent endocytic pathways have been described in other eukaryotes. Here, we provide evidence for a clathrin-independent endocytic pathway in yeast. In cells lacking the clathrin-binding adaptor proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2. This second pathway does not require components of the better-studied endocytic pathway, including clathrin and Arp2/3 complex activators. Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells.
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spelling pubmed-32575292012-05-14 Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin Prosser, Derek C. Drivas, Theodore G. Maldonado-Báez, Lymarie Wendland, Beverly J Cell Biol Research Articles Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrin-independent endocytic pathways have been described in other eukaryotes. Here, we provide evidence for a clathrin-independent endocytic pathway in yeast. In cells lacking the clathrin-binding adaptor proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2. This second pathway does not require components of the better-studied endocytic pathway, including clathrin and Arp2/3 complex activators. Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells. The Rockefeller University Press 2011-11-14 /pmc/articles/PMC3257529/ /pubmed/22065638 http://dx.doi.org/10.1083/jcb.201104045 Text en © 2011 Prosser et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Prosser, Derek C.
Drivas, Theodore G.
Maldonado-Báez, Lymarie
Wendland, Beverly
Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title_full Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title_fullStr Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title_full_unstemmed Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title_short Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin
title_sort existence of a novel clathrin-independent endocytic pathway in yeast that depends on rho1 and formin
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257529/
https://www.ncbi.nlm.nih.gov/pubmed/22065638
http://dx.doi.org/10.1083/jcb.201104045
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