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Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis
In many organisms, the small guanosine triphosphatase RhoA controls assembly and contraction of the actomyosin ring during cytokinesis by activating different effectors. Although the role of some RhoA effectors like formins and Rho kinase is reasonably understood, the functions of another putative e...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257531/ https://www.ncbi.nlm.nih.gov/pubmed/22084308 http://dx.doi.org/10.1083/jcb.201105136 |
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author | Bassi, Zuni I. Verbrugghe, Koen J. Capalbo, Luisa Gregory, Stephen Montembault, Emilie Glover, David M. D’Avino, Pier Paolo |
author_facet | Bassi, Zuni I. Verbrugghe, Koen J. Capalbo, Luisa Gregory, Stephen Montembault, Emilie Glover, David M. D’Avino, Pier Paolo |
author_sort | Bassi, Zuni I. |
collection | PubMed |
description | In many organisms, the small guanosine triphosphatase RhoA controls assembly and contraction of the actomyosin ring during cytokinesis by activating different effectors. Although the role of some RhoA effectors like formins and Rho kinase is reasonably understood, the functions of another putative effector, Citron kinase (CIT-K), are still debated. In this paper, we show that, contrary to previous models, the Drosophila melanogaster CIT-K orthologue Sticky (Sti) does not require interaction with RhoA to localize to the cleavage site. Instead, RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. Moreover, we found that the Sti Citron-Nik1 homology domain interacts with RhoA regardless of its status, indicating that Sti is not a canonical RhoA effector. Finally, Sti depletion caused an increase of phosphorylated myosin regulatory light chain at the cleavage site in late cytokinesis. We propose that Sti/CIT-K maintains correct RhoA localization at the cleavage site, which is necessary for proper RhoA activity and contractile ring dynamics. |
format | Online Article Text |
id | pubmed-3257531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32575312012-05-14 Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis Bassi, Zuni I. Verbrugghe, Koen J. Capalbo, Luisa Gregory, Stephen Montembault, Emilie Glover, David M. D’Avino, Pier Paolo J Cell Biol Research Articles In many organisms, the small guanosine triphosphatase RhoA controls assembly and contraction of the actomyosin ring during cytokinesis by activating different effectors. Although the role of some RhoA effectors like formins and Rho kinase is reasonably understood, the functions of another putative effector, Citron kinase (CIT-K), are still debated. In this paper, we show that, contrary to previous models, the Drosophila melanogaster CIT-K orthologue Sticky (Sti) does not require interaction with RhoA to localize to the cleavage site. Instead, RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. Moreover, we found that the Sti Citron-Nik1 homology domain interacts with RhoA regardless of its status, indicating that Sti is not a canonical RhoA effector. Finally, Sti depletion caused an increase of phosphorylated myosin regulatory light chain at the cleavage site in late cytokinesis. We propose that Sti/CIT-K maintains correct RhoA localization at the cleavage site, which is necessary for proper RhoA activity and contractile ring dynamics. The Rockefeller University Press 2011-11-14 /pmc/articles/PMC3257531/ /pubmed/22084308 http://dx.doi.org/10.1083/jcb.201105136 Text en © 2011 Bassi et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Bassi, Zuni I. Verbrugghe, Koen J. Capalbo, Luisa Gregory, Stephen Montembault, Emilie Glover, David M. D’Avino, Pier Paolo Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title | Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title_full | Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title_fullStr | Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title_full_unstemmed | Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title_short | Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis |
title_sort | sticky/citron kinase maintains proper rhoa localization at the cleavage site during cytokinesis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257531/ https://www.ncbi.nlm.nih.gov/pubmed/22084308 http://dx.doi.org/10.1083/jcb.201105136 |
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