Cargando…

Identification of host cell factors required for intoxication through use of modified cholera toxin

We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to...

Descripción completa

Detalles Bibliográficos
Autores principales: Guimaraes, Carla P., Carette, Jan E., Varadarajan, Malini, Antos, John, Popp, Maximilian W., Spooner, Eric, Brummelkamp, Thijn R., Ploegh, Hidde L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257576/
https://www.ncbi.nlm.nih.gov/pubmed/22123862
http://dx.doi.org/10.1083/jcb.201108103