Cargando…
Identification of host cell factors required for intoxication through use of modified cholera toxin
We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to...
Autores principales: | Guimaraes, Carla P., Carette, Jan E., Varadarajan, Malini, Antos, John, Popp, Maximilian W., Spooner, Eric, Brummelkamp, Thijn R., Ploegh, Hidde L. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257576/ https://www.ncbi.nlm.nih.gov/pubmed/22123862 http://dx.doi.org/10.1083/jcb.201108103 |
Ejemplares similares
-
A Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κB
por: Lee, Clarissa C., et al.
Publicado: (2013) -
Global gene disruption in human cells to assign genes to phenotypes
por: Carette, Jan E., et al.
Publicado: (2011) -
GM1 ganglioside-independent intoxication by Cholera toxin
por: Cervin, Jakob, et al.
Publicado: (2018) -
Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity
por: Antos, John M., et al.
Publicado: (2009) -
A Therapeutic Chemical Chaperone Inhibits Cholera Intoxication and Unfolding/Translocation of the Cholera Toxin A1 Subunit
por: Taylor, Michael, et al.
Publicado: (2011)