Cargando…

Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.

The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides,...

Descripción completa

Detalles Bibliográficos
Autores principales: Armstrong, J, Smeekens, S, Rottier, P
Formato: Texto
Lenguaje:English
Publicado: 1983
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC325759/
https://www.ncbi.nlm.nih.gov/pubmed/6687635
_version_ 1782121178644611072
author Armstrong, J
Smeekens, S
Rottier, P
author_facet Armstrong, J
Smeekens, S
Rottier, P
author_sort Armstrong, J
collection PubMed
description The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues.
format Text
id pubmed-325759
institution National Center for Biotechnology Information
language English
publishDate 1983
record_format MEDLINE/PubMed
spelling pubmed-3257592004-02-10 Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. Armstrong, J Smeekens, S Rottier, P Nucleic Acids Res The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues. 1983-02-11 /pmc/articles/PMC325759/ /pubmed/6687635 Text en
spellingShingle Armstrong, J
Smeekens, S
Rottier, P
Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title_full Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title_fullStr Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title_full_unstemmed Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title_short Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
title_sort sequence of the nucleocapsid gene from murine coronavirus mhv-a59.
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC325759/
https://www.ncbi.nlm.nih.gov/pubmed/6687635
work_keys_str_mv AT armstrongj sequenceofthenucleocapsidgenefrommurinecoronavirusmhva59
AT smeekenss sequenceofthenucleocapsidgenefrommurinecoronavirusmhva59
AT rottierp sequenceofthenucleocapsidgenefrommurinecoronavirusmhva59