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Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.
The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides,...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1983
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC325759/ https://www.ncbi.nlm.nih.gov/pubmed/6687635 |
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author | Armstrong, J Smeekens, S Rottier, P |
author_facet | Armstrong, J Smeekens, S Rottier, P |
author_sort | Armstrong, J |
collection | PubMed |
description | The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues. |
format | Text |
id | pubmed-325759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
record_format | MEDLINE/PubMed |
spelling | pubmed-3257592004-02-10 Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. Armstrong, J Smeekens, S Rottier, P Nucleic Acids Res The nucleotide sequence of the RNA encoding the nucleocapsid protein of coronavirus MHV-A59 has been determined. Copy DNA was prepared from mRNA isolated from virally infected cells, fragmented and cloned in the phage vector M13 mp8 for direct sequence determination. A sequence of 1817 nucleotides, adjacent to the viral poly-A tail, was obtained. It contains a single long open reading frame encoding a protein of mol. wt. 49660, which is enriched in basic residues. 1983-02-11 /pmc/articles/PMC325759/ /pubmed/6687635 Text en |
spellingShingle | Armstrong, J Smeekens, S Rottier, P Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title | Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title_full | Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title_fullStr | Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title_full_unstemmed | Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title_short | Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. |
title_sort | sequence of the nucleocapsid gene from murine coronavirus mhv-a59. |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC325759/ https://www.ncbi.nlm.nih.gov/pubmed/6687635 |
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