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Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism

Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans. We have identified protein NE81 in Dictyostelium, which has properti...

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Autores principales: Krüger, Anne, Batsios, Petros, Baumann, Otto, Luckert, Eva, Schwarz, Heinz, Stick, Reimer, Meyer, Irene, Gräf, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258179/
https://www.ncbi.nlm.nih.gov/pubmed/22090348
http://dx.doi.org/10.1091/mbc.E11-07-0595
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author Krüger, Anne
Batsios, Petros
Baumann, Otto
Luckert, Eva
Schwarz, Heinz
Stick, Reimer
Meyer, Irene
Gräf, Ralph
author_facet Krüger, Anne
Batsios, Petros
Baumann, Otto
Luckert, Eva
Schwarz, Heinz
Stick, Reimer
Meyer, Irene
Gräf, Ralph
author_sort Krüger, Anne
collection PubMed
description Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans. We have identified protein NE81 in Dictyostelium, which has properties that justify its denomination as a lamin-like protein in a lower eukaryote. This is based on its primary structure, subcellular localization, and regulation during mitosis, and its requirement of the C-terminal CaaX box as a posttranslational processing signal for proper localization. Our knockout and overexpression mutants revealed an important role for NE81 in nuclear integrity, chromatin organization, and mechanical stability of cells. All our results are in agreement with a role for NE81 in formation of a nuclear lamina. This function is corroborated by localization of Dictyostelium NE81 at the nuclear envelope in human cells. The discovery of a lamin-like protein in a unicellular organism is not only intriguing in light of evolution, it may also provide a simple experimental platform for studies of the molecular basis of laminopathies.
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spelling pubmed-32581792012-03-30 Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism Krüger, Anne Batsios, Petros Baumann, Otto Luckert, Eva Schwarz, Heinz Stick, Reimer Meyer, Irene Gräf, Ralph Mol Biol Cell Articles Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans. We have identified protein NE81 in Dictyostelium, which has properties that justify its denomination as a lamin-like protein in a lower eukaryote. This is based on its primary structure, subcellular localization, and regulation during mitosis, and its requirement of the C-terminal CaaX box as a posttranslational processing signal for proper localization. Our knockout and overexpression mutants revealed an important role for NE81 in nuclear integrity, chromatin organization, and mechanical stability of cells. All our results are in agreement with a role for NE81 in formation of a nuclear lamina. This function is corroborated by localization of Dictyostelium NE81 at the nuclear envelope in human cells. The discovery of a lamin-like protein in a unicellular organism is not only intriguing in light of evolution, it may also provide a simple experimental platform for studies of the molecular basis of laminopathies. The American Society for Cell Biology 2012-01-15 /pmc/articles/PMC3258179/ /pubmed/22090348 http://dx.doi.org/10.1091/mbc.E11-07-0595 Text en © 2012 Krüger et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Krüger, Anne
Batsios, Petros
Baumann, Otto
Luckert, Eva
Schwarz, Heinz
Stick, Reimer
Meyer, Irene
Gräf, Ralph
Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title_full Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title_fullStr Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title_full_unstemmed Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title_short Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
title_sort characterization of ne81, the first lamin-like nucleoskeleton protein in a unicellular organism
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258179/
https://www.ncbi.nlm.nih.gov/pubmed/22090348
http://dx.doi.org/10.1091/mbc.E11-07-0595
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