Cargando…

Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

BACKGROUND: Next generation sequencing (NGS) enables a more comprehensive analysis of bacterial diversity from complex environmental samples. NGS data can be analysed using a variety of workflows. We test several simple and complex workflows, including frequently used as well as recently published t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Barriuso, Jorge, Valverde , Jose R, Mellado, Rafael P
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258296/ https://www.ncbi.nlm.nih.gov/pubmed/22168258 http://dx.doi.org/10.1186/1471-2105-12-473

Ejemplares similares

Identification of Leptotrichia goodfellowii infective endocarditis by next-generation sequencing of 16S rDNA amplicons
por: Lieberman, Joshua A., et al.
Publicado: (2021)

Microbiota analysis with next-generation 16S rDNA gene sequencing in recurrent common bile duct stones
por: Tan, Wenhui, et al.
Publicado: (2022)

Benchmarking and Validation of a Bioinformatics Workflow for Meat Species Identification Using 16S rDNA Metabarcoding
por: Denay, Grégoire, et al.
Publicado: (2023)

Bacterial 16S rRNA/rDNA Profiling in the Liquid Phase of Human Saliva
por: Gu, F, et al.
Publicado: (2009)

Diversity of Intestinal Bacterial Microbiota of Indigenous and Commercial Strains of Chickens Using 16S rDNA-Based Analysis
por: Al-Marzooqi, Waleed, et al.
Publicado: (2020)

Bacterial diversity in primary infected root canals of a Chinese cohort: analysis of 16 S rDNA sequencing
por: Hu, Ziqiu, et al.
Publicado: (2023)

16S rDNA Pyrosequencing Analysis of Bacterial Community in Heavy Metals Polluted Soils
por: Gołębiewski, Marcin, et al.
Publicado: (2014)

Investigating bacterial populations in styrene-degrading biofilters by 16S rDNA tag pyrosequencing
por: Portune, Kevin J., et al.
Publicado: (2014)

16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections
por: Ziegler, Ingrid, et al.
Publicado: (2019)

Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing
por: Burton, Aaron S., et al.
Publicado: (2020)

Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)
por: Dowd, Scot E, et al.
Publicado: (2008)

Effects of 16S rDNA sampling on estimates of the number of endosymbiont lineages in sucking lice
por: Allen, Julie M., et al.
Publicado: (2016)

Microbial Diversity of Bovine Mastitic Milk as Described by Pyrosequencing of Metagenomic 16s rDNA
por: Oikonomou, Georgios, et al.
Publicado: (2012)

Molecular analysis of the mitochondrial markers COI, 12S rDNA and 16S rDNA for six species of Iranian scorpions
por: Soltan-Alinejad, Parisa, et al.
Publicado: (2021)

Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids
por: Matyášek, Roman, et al.
Publicado: (2012)

Assessing Symbiodinium diversity in scleractinian corals via next-generation sequencing-based genotyping of the ITS2 rDNA region
por: Arif, Chatchanit, et al.
Publicado: (2014)

Comparison of 16S rDNA-PCR Amplification and Culture of Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis
por: Sarookhani, Mohammad-Reza, et al.
Publicado: (2010)

Characterization of Bacterial Communities in Selected Smokeless Tobacco Products Using 16S rDNA Analysis
por: Tyx, Robert E., et al.
Publicado: (2016)

Metagenomic 16S rDNA amplicon data on bacterial diversity profiling and its predicted metabolic functions of varillales in Allpahuayo-Mishana National Reserve
por: Castro, Juan C., et al.
Publicado: (2020)

Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons
por: Siddiqui, Huma, et al.
Publicado: (2011)

Analysis of microbial diversity in apple vinegar fermentation process through 16s rDNA sequencing
por: Song, Juan, et al.
Publicado: (2019)

Fate of Functional Bacterial and Eukaryotic Community Regulated by Earthworms during Vermicomposting of Dewatered Sludge, Studies Based on the 16S rDNA and 18S rDNA Sequencing of Active Cells
por: Yang, Jun, et al.
Publicado: (2021)

Do calcifying nanoparticles really contain 16S rDNA?
por: Shiekh, Farooq A
Publicado: (2012)

16S rDNA analysis of the intestinal microbes in osteoporotic rats
por: SUN, Yan, et al.
Publicado: (2021)

Assessment of four DNA fragments (COI, 16S rDNA, ITS2, 12S rDNA) for species identification of the Ixodida (Acari: Ixodida)
por: Lv, Jizhou, et al.
Publicado: (2014)

Performance of 16s rDNA Primer Pairs in the Study of Rhizosphere and Endosphere Bacterial Microbiomes in Metabarcoding Studies
por: Beckers, Bram, et al.
Publicado: (2016)

Identification of causative pathogens in mouse eyes with bacterial keratitis by sequence analysis of 16S rDNA libraries
por: Song, Hong-Yan, et al.
Publicado: (2014)

Determination of uterine bacterial community in postpartum dairy cows with metritis based on 16S rDNA sequencing
por: Chen, Hao, et al.
Publicado: (2020)

Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis
por: Cariou, Marie, et al.
Publicado: (2018)

Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing
por: Xu, Linfen, et al.
Publicado: (2020)

Calorie restriction reduces rDNA recombination independently of rDNA silencing
por: Riesen, Michèle, et al.
Publicado: (2009)

Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium
por: Kalia, Vipin Chandra, et al.
Publicado: (2011)

Microbiota in Human Periodontal Abscess Revealed by 16S rDNA Sequencing
por: Chen, Jiazhen, et al.
Publicado: (2019)

16S rDNA sequencing and metadata of Dutch dental unit water
por: Hoogenkamp, Michel A., et al.
Publicado: (2021)

Bacterial communities 16S rDNA fingerprinting as a potential tracing tool for cultured seabass Dicentrarchus labrax
por: Pimentel, Tânia, et al.
Publicado: (2017)

Comparison of 16S rDNA Amplicon Sequencing With the Culture Method for Diagnosing Causative Pathogens in Bacterial Corneal Infections
por: An, Na, et al.
Publicado: (2022)

Significant Short-Term Shifts in the Microbiomes of Smokers With Periodontitis After Periodontal Therapy With Amoxicillin & Metronidazole as Revealed by 16S rDNA Amplicon Next Generation Sequencing
por: Hagenfeld, Daniel, et al.
Publicado: (2020)

Quelling targets the rDNA locus and functions in rDNA copy number control
por: Cecere, Germano, et al.
Publicado: (2009)

In vivo Microbial Diversity Analysis on Different Surfaces of Dental Restorative Materials via 16S rDNA Sequencing
por: Tu, Yan, et al.
Publicado: (2020)

Bacterial Diversity and Community Structure in the Pine Wood Nematode Bursaphelenchus xylophilus and B. mucronatus with Different Virulence by High-Throughput Sequencing of the 16S rDNA
por: Xiang, Yang, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_sdfsosga3igoh0b96atvnnik1e