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The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots

The main effects of antagonistic rhizobacteria on plant pathogenic fungi are antibiosis, fungistasis or an indirect constraint through the induction of a plant defence response. To explore different biocontrol mechanisms, an in vitro confrontation assay was conducted with the rhizobacterium Pseudomo...

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Autores principales: DAVAL, STÉPHANIE, LEBRETON, LIONEL, GAZENGEL, KÉVIN, BOUTIN, MORGANE, GUILLERM‐ERCKELBOUDT, ANNE‐YVONNE, SARNIGUET, ALAIN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258481/
https://www.ncbi.nlm.nih.gov/pubmed/21726382
http://dx.doi.org/10.1111/j.1364-3703.2011.00715.x
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author DAVAL, STÉPHANIE
LEBRETON, LIONEL
GAZENGEL, KÉVIN
BOUTIN, MORGANE
GUILLERM‐ERCKELBOUDT, ANNE‐YVONNE
SARNIGUET, ALAIN
author_facet DAVAL, STÉPHANIE
LEBRETON, LIONEL
GAZENGEL, KÉVIN
BOUTIN, MORGANE
GUILLERM‐ERCKELBOUDT, ANNE‐YVONNE
SARNIGUET, ALAIN
author_sort DAVAL, STÉPHANIE
collection PubMed
description The main effects of antagonistic rhizobacteria on plant pathogenic fungi are antibiosis, fungistasis or an indirect constraint through the induction of a plant defence response. To explore different biocontrol mechanisms, an in vitro confrontation assay was conducted with the rhizobacterium Pseudomonas fluorescens Pf29Arp as a biocontrol agent of the fungus Gaeumannomyces graminis var. tritici (Ggt) on wheat roots. In parallel with the assessment of disease extension, together with the bacterial and fungal root colonization rates, the transcript levels of candidate fungal pathogenicity and plant‐induced genes were monitored during the 10‐day infection process. The bacterial inoculation of wheat roots with the Pf29Arp strain reduced the development of Ggt‐induced disease expressed as attack frequency and necrosis length. The growth rates of Ggt and Pf29Arp, monitored through quantitative polymerase chain reaction of DNA amounts with a part of the Ggt 18S rDNA gene and a specific Pf29Arp strain detection probe, respectively, increased throughout the interactions. Bacterial antagonism and colonization had no significant effect on root colonization by Ggt. The expression of fungal and plant genes was quantified in planta by quantitative reverse transcription‐polymerase chain reaction during the interactions thanks to the design of specific primers and an innovative universal reference system. During the early stages of the tripartite interaction, several of the fungal genes assayed were down‐regulated by Pf29Arp, including two laccases, a β‐1,3‐exoglucanase and a mitogen‐activated protein kinase. The plant host glutathione‐S‐transferase gene was induced by Ggt alone and up‐regulated by Pf29Arp bacteria in interaction with the pathogen. We conclude that Pf29Arp antagonism acts through the alteration of fungal pathogenesis and probably through the activation of host defences.
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spelling pubmed-32584812012-01-17 The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots DAVAL, STÉPHANIE LEBRETON, LIONEL GAZENGEL, KÉVIN BOUTIN, MORGANE GUILLERM‐ERCKELBOUDT, ANNE‐YVONNE SARNIGUET, ALAIN Mol Plant Pathol Original Articles The main effects of antagonistic rhizobacteria on plant pathogenic fungi are antibiosis, fungistasis or an indirect constraint through the induction of a plant defence response. To explore different biocontrol mechanisms, an in vitro confrontation assay was conducted with the rhizobacterium Pseudomonas fluorescens Pf29Arp as a biocontrol agent of the fungus Gaeumannomyces graminis var. tritici (Ggt) on wheat roots. In parallel with the assessment of disease extension, together with the bacterial and fungal root colonization rates, the transcript levels of candidate fungal pathogenicity and plant‐induced genes were monitored during the 10‐day infection process. The bacterial inoculation of wheat roots with the Pf29Arp strain reduced the development of Ggt‐induced disease expressed as attack frequency and necrosis length. The growth rates of Ggt and Pf29Arp, monitored through quantitative polymerase chain reaction of DNA amounts with a part of the Ggt 18S rDNA gene and a specific Pf29Arp strain detection probe, respectively, increased throughout the interactions. Bacterial antagonism and colonization had no significant effect on root colonization by Ggt. The expression of fungal and plant genes was quantified in planta by quantitative reverse transcription‐polymerase chain reaction during the interactions thanks to the design of specific primers and an innovative universal reference system. During the early stages of the tripartite interaction, several of the fungal genes assayed were down‐regulated by Pf29Arp, including two laccases, a β‐1,3‐exoglucanase and a mitogen‐activated protein kinase. The plant host glutathione‐S‐transferase gene was induced by Ggt alone and up‐regulated by Pf29Arp bacteria in interaction with the pathogen. We conclude that Pf29Arp antagonism acts through the alteration of fungal pathogenesis and probably through the activation of host defences. Blackwell Publishing Ltd 2011-04-21 /pmc/articles/PMC3258481/ /pubmed/21726382 http://dx.doi.org/10.1111/j.1364-3703.2011.00715.x Text en © 2011 The Authors. Molecular Plant Pathology © 2011 BSPP and Blackwell Publishing Ltd Open access.
spellingShingle Original Articles
DAVAL, STÉPHANIE
LEBRETON, LIONEL
GAZENGEL, KÉVIN
BOUTIN, MORGANE
GUILLERM‐ERCKELBOUDT, ANNE‐YVONNE
SARNIGUET, ALAIN
The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title_full The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title_fullStr The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title_full_unstemmed The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title_short The biocontrol bacterium Pseudomonas fluorescens Pf29Arp strain affects the pathogenesis‐related gene expression of the take‐all fungus Gaeumannomyces graminis var. tritici on wheat roots
title_sort biocontrol bacterium pseudomonas fluorescens pf29arp strain affects the pathogenesis‐related gene expression of the take‐all fungus gaeumannomyces graminis var. tritici on wheat roots
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258481/
https://www.ncbi.nlm.nih.gov/pubmed/21726382
http://dx.doi.org/10.1111/j.1364-3703.2011.00715.x
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