Cargando…

Rapid Detection of rpoB Mutations in Rifampin Resistant M. tuberculosis from Sputum Samples by Denaturing Gradient Gel Electrophoresis

Objective: To establish a rapid detection method for identifying rpoB mutations associated with rifampin (RIF) resistance in sputum specimens. Methods: We detected rpoB mutations directly in 90 sputum specimens collected from suspected tuberculosis patients using PCR-based denaturing gradient gel el...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Jun, Xin, Jiaojiao, Zhang, Liyuan, Jiang, Longyan, Cao, Hongcui, Li, Lanjuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Ivyspring International Publisher 2012
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258557/ https://www.ncbi.nlm.nih.gov/pubmed/22253562 http://dx.doi.org/10.7150/ijms.3605

Ejemplares similares

rpoB Mutations and Effects on Rifampin Resistance in Mycobacterium tuberculosis
por: Li, Ma-chao, et al.
Publicado: (2021)

rpoB Mutations are Associated with Variable Levels of Rifampin and Rifabutin Resistance in Mycobacterium tuberculosis
por: Li, Ma-Chao, et al.
Publicado: (2022)

Genetic evaluation of relationship between mutations in rpoB and resistance of Mycobacterium tuberculosis to rifampin
por: Zaczek, Anna, et al.
Publicado: (2009)

Few amino acid positions in rpoB are associated with most of the rifampin resistance in Mycobacterium tuberculosis
por: Cummings, Michael P, et al.
Publicado: (2004)

Investigation of the rpoB Mutations Causing Rifampin Resistance by Rapid Screening in Mycobacterium Tuberculosis in North-East of Iran
por: Tajbakhsh, Amir, et al.
Publicado: (2018)

rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis isolates from rural areas of Zhejiang, China
por: Zeng, Mei-Chun, et al.
Publicado: (2021)

Determination of Potentially Novel Compensatory Mutations in rpoC Associated with Rifampin Resistance and rpoB Mutations in Mycobacterium tuberculosis Clinical Isolates from Peru
por: Vargas, Ana Paula, et al.
Publicado: (2020)

Successful Treatment with a High-dose Rifampin-containing Regimen for Pulmonary Tuberculosis with a Disputed rpoB Mutation
por: Jeong, Dae Hyun, et al.
Publicado: (2018)

Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
por: Wu, Dachang, et al.
Publicado: (2014)

Borderline rpoB mutations transmit at the same rate as common rpoB mutations in a tuberculosis cohort in Bangladesh
por: Lempens, Pauline, et al.
Publicado: (2023)

Detection of FCoV quasispecies using denaturing gradient gel electrophoresis
por: Gunn-Moore, D.A, et al.
Publicado: (1999)

rpoB Gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism.
por: Bobadilla-del-Valle, M, et al.
Publicado: (2001)

Comparative Analysis of Denaturing Gradient Gel Electrophoresis and Temporal Temperature Gradient Gel Electrophoresis Profiles as a Tool for the Differentiation of Candida Species
por: Mohammadi, Parisa, et al.
Publicado: (2015)

Correlation of rpoB Mutations with Minimal Inhibitory Concentration of Rifampin and Rifabutin in Mycobacterium tuberculosis in an HIV/AIDS Endemic Setting, South Africa
por: Rukasha, Ivy, et al.
Publicado: (2016)

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB, and nested-rpoB PCR Cloning
por: Meghdadi, Hossein, et al.
Publicado: (2015)

Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma
por: Vael, Carl, et al.
Publicado: (2011)

Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
por: Guo, Yinjuan, et al.
Publicado: (2021)

Differential Impact of the rpoB Mutant on Rifampin and Rifabutin Resistance Signatures of Mycobacterium tuberculosis Is Revealed Using a Whole-Genome Sequencing Assay
por: Yu, Ming-Chih, et al.
Publicado: (2022)

Multiple rpoB Mutants of Mycobacterium tuberculosis and Second-order Selection
por: Mokrousov, Igor
Publicado: (2004)

Use of denaturing-gradient gel electrophoresis to study chromium-induced point mutations in human cells.
por: Chen, J, et al.
Publicado: (1994)

Application of Nested PCR-DGGE (Denaturing Gradient Gel Electrophoresis) for the Analysis of Ciliate Communities in Soils
por: Shimano, Satoshi, et al.
Publicado: (2012)

PCR-denaturing Gradient Gel Electrophoresis as a Simple Identification Tool of Arbuscular Mycorrhizal Fungal Isolates
por: Ohtomo, Ryo, et al.
Publicado: (2019)

Rifabutin Resistance Associated with Double Mutations in rpoB Gene in Mycobacterium tuberculosis Isolates
por: Jing, Wei, et al.
Publicado: (2017)

A Glutamine Insertion at Codon 432 of RpoB Confers Rifampicin Resistance in Mycobacterium tuberculosis
por: Lai, Li-Yin, et al.
Publicado: (2020)

Complexity of vaginal microflora as analyzed by PCR denaturing gradient gel electrophoresis in a patient with recurrent bacterial vaginosis.
por: Devillard, Estelle, et al.
Publicado: (2005)

Denaturing Gradient Gel Electrophoresis (DGGE) as a Powerful Novel Alternative for Differentiation of Epizootic ISA Virus Variants
por: Carmona, Marisela, et al.
Publicado: (2012)

Design and Evaluation of PCR Primers for Denaturing Gradient Gel Electrophoresis Analysis of Plant Parasitic and Fungivorous Nematode Communities
por: Kushida, Atsuhiko
Publicado: (2013)

16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances
por: Piwat, S., et al.
Publicado: (2013)

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
por: López-López, P., et al.
Publicado: (2017)

Commentary: Rifabutin Resistance Associated with Double Mutations in rpoB Gene in Mycobacterium tuberculosis Isolates
por: Sharma, Sandeep, et al.
Publicado: (2017)

Frequency and Type of Disputed rpoB Mutations in Mycobacterium tuberculosis Isolates from South Korea
por: Jo, Kyung-Wook, et al.
Publicado: (2017)

HIV Coinfection Is Associated with Low-Fitness rpoB Variants in Rifampicin-Resistant Mycobacterium tuberculosis
por: Loiseau, Chloé, et al.
Publicado: (2020)

Compensatory effects of M. tuberculosis rpoB mutations outside the rifampicin resistance-determining region
por: Ma, Pengjiao, et al.
Publicado: (2021)

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
por: Halfmann, Randal, et al.
Publicado: (2008)

Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene
por: Li, Baiyuan, et al.
Publicado: (2014)

Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis
por: Xiong, Xiong, et al.
Publicado: (2018)

Variable ability of rapid tests to detect Mycobacterium tuberculosis rpoB mutations conferring phenotypically occult rifampicin resistance
por: Torrea, Gabriela, et al.
Publicado: (2019)

Molecular characterization of rpoB gene mutations in isolates from tuberculosis patients in Cubal, Republic of Angola
por: Rando-Segura, Ariadna, et al.
Publicado: (2021)

The Structural Basis of Mycobacterium tuberculosis RpoB Drug-Resistant Clinical Mutations on Rifampicin Drug Binding
por: Amusengeri, Arnold, et al.
Publicado: (2022)

GeneXpert MTB/RIF Ultra performance to detect uncommon rpoB mutations in Mycobacterium tuberculosis
por: Rigouts, Leen, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_vme4dvnfv6c49bln4gb8gfk3tl