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Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney

INTRODUCTION: Transforming growth factor-β1 (TGF-β1) has a crucial role in collagen synthesis and fibrosis. TGF-β1 can be antagonized and/or reduced by the action of certain agents. We propose to identify the role of decorin in treatment of tubular and interstitial fibrosis and in the inhibition of...

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Autores principales: Alan, Cabir, Kocoglu, Hasan, AltIntas, Ramazan, AlIcI, Bülent, Resit Ersay, Ahmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258707/
https://www.ncbi.nlm.nih.gov/pubmed/22291758
http://dx.doi.org/10.5114/aoms.2011.22069
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author Alan, Cabir
Kocoglu, Hasan
AltIntas, Ramazan
AlIcI, Bülent
Resit Ersay, Ahmet
author_facet Alan, Cabir
Kocoglu, Hasan
AltIntas, Ramazan
AlIcI, Bülent
Resit Ersay, Ahmet
author_sort Alan, Cabir
collection PubMed
description INTRODUCTION: Transforming growth factor-β1 (TGF-β1) has a crucial role in collagen synthesis and fibrosis. TGF-β1 can be antagonized and/or reduced by the action of certain agents. We propose to identify the role of decorin in treatment of tubular and interstitial fibrosis and in the inhibition of TGF-β1 in an acute ischaemic kidney. MATERIAL AND METHODS: We grouped 34 female Sprague Dawley type rats into 3 groups as 9 sham, 9 ischaemia-reperfusion (I/R) and 16 I/R + decorin respectively. The rats in the I/R + decorin group had decorin administered intraperitoneally at the dose of 0.1 mg/kg for 9 days after reperfusion. After 9 days, all the rats in the 3 groups were unilaterally nephrectomized. The TGF-β1 level was measured immunohistochemically in the nephrectomized material. RESULTS: The TGF-β1 level was lower in the I/R + decorin group. Evaluation of apoptotic activity level by caspase staining showed a statistically significant difference between the 3 groups. The number of caspase stained cells was lower in the I/R + decorin group. The amount of collagen in interstitial tissue was higher in the I/R group than in the I/R + decorin group, but this difference was not statistically significant. CONCLUSIONS: We found that the TGF-β1 level – the so-called initiator of fibrotic activity – and apoptotic activity were low in the I/R + decorin group. Additional studies must be performed to understand the role of decorin in inhibition of TGF-β1 and to assess decorin’s routine use in acute renal ischaemia.
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spelling pubmed-32587072012-01-30 Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney Alan, Cabir Kocoglu, Hasan AltIntas, Ramazan AlIcI, Bülent Resit Ersay, Ahmet Arch Med Sci Basic Research INTRODUCTION: Transforming growth factor-β1 (TGF-β1) has a crucial role in collagen synthesis and fibrosis. TGF-β1 can be antagonized and/or reduced by the action of certain agents. We propose to identify the role of decorin in treatment of tubular and interstitial fibrosis and in the inhibition of TGF-β1 in an acute ischaemic kidney. MATERIAL AND METHODS: We grouped 34 female Sprague Dawley type rats into 3 groups as 9 sham, 9 ischaemia-reperfusion (I/R) and 16 I/R + decorin respectively. The rats in the I/R + decorin group had decorin administered intraperitoneally at the dose of 0.1 mg/kg for 9 days after reperfusion. After 9 days, all the rats in the 3 groups were unilaterally nephrectomized. The TGF-β1 level was measured immunohistochemically in the nephrectomized material. RESULTS: The TGF-β1 level was lower in the I/R + decorin group. Evaluation of apoptotic activity level by caspase staining showed a statistically significant difference between the 3 groups. The number of caspase stained cells was lower in the I/R + decorin group. The amount of collagen in interstitial tissue was higher in the I/R group than in the I/R + decorin group, but this difference was not statistically significant. CONCLUSIONS: We found that the TGF-β1 level – the so-called initiator of fibrotic activity – and apoptotic activity were low in the I/R + decorin group. Additional studies must be performed to understand the role of decorin in inhibition of TGF-β1 and to assess decorin’s routine use in acute renal ischaemia. Termedia Publishing House 2011-04 2011-05-17 /pmc/articles/PMC3258707/ /pubmed/22291758 http://dx.doi.org/10.5114/aoms.2011.22069 Text en Copyright © 2011 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Alan, Cabir
Kocoglu, Hasan
AltIntas, Ramazan
AlIcI, Bülent
Resit Ersay, Ahmet
Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title_full Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title_fullStr Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title_full_unstemmed Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title_short Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
title_sort protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258707/
https://www.ncbi.nlm.nih.gov/pubmed/22291758
http://dx.doi.org/10.5114/aoms.2011.22069
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