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Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer
INTRODUCTION: The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer. MATERIAL AND METHODS: Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258754/ https://www.ncbi.nlm.nih.gov/pubmed/22295037 http://dx.doi.org/10.5114/aoms.2011.23421 |
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author | Jabłonowski, Zbigniew Reszka, Edyta Gromadzińska, Jolanta Wąsowicz, Wojciech Sosnowski, Marek |
author_facet | Jabłonowski, Zbigniew Reszka, Edyta Gromadzińska, Jolanta Wąsowicz, Wojciech Sosnowski, Marek |
author_sort | Jabłonowski, Zbigniew |
collection | PubMed |
description | INTRODUCTION: The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer. MATERIAL AND METHODS: Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls were studied. Isolation of genomic DNA from blood serum and methylation-specific PCR (MSP) were applied. Methylation status – methylated and unmethylated promoter regions of p16 and DAPK genes were analysed. RESULTS: Seventeen out of 42 patients (40.5%) had the methylated p16 gene, while methylation of the DAPK gene was seen in 27 of 42 cases (64.3%). In 12 patients (28.6%) both analysed genes were methylated. A statistically significant (p = 0.046) higher frequency of DAPK gene methylation (71.4%) was observed in patients with lower grade (G1) bladder cancer. CONCLUSIONS: Detection of the aberrant hypermethylation of DAPK and p16 genes in blood DNA from non-invasive bladder cancer patients might offer an effective means for earlier auxiliary diagnosis of the malignancy. |
format | Online Article Text |
id | pubmed-3258754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-32587542012-01-31 Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer Jabłonowski, Zbigniew Reszka, Edyta Gromadzińska, Jolanta Wąsowicz, Wojciech Sosnowski, Marek Arch Med Sci Clinical Research INTRODUCTION: The aim of the study was to examine the frequency of methylation status in promoter regions of p16 and DAPK genes in patients with non-invasive bladder cancer. MATERIAL AND METHODS: Forty-two patients (92.9% men, 73.8% smokers, 71.4% T1G1, 19.1% T1G2, 9.5% T1G3) and 36 healthy controls were studied. Isolation of genomic DNA from blood serum and methylation-specific PCR (MSP) were applied. Methylation status – methylated and unmethylated promoter regions of p16 and DAPK genes were analysed. RESULTS: Seventeen out of 42 patients (40.5%) had the methylated p16 gene, while methylation of the DAPK gene was seen in 27 of 42 cases (64.3%). In 12 patients (28.6%) both analysed genes were methylated. A statistically significant (p = 0.046) higher frequency of DAPK gene methylation (71.4%) was observed in patients with lower grade (G1) bladder cancer. CONCLUSIONS: Detection of the aberrant hypermethylation of DAPK and p16 genes in blood DNA from non-invasive bladder cancer patients might offer an effective means for earlier auxiliary diagnosis of the malignancy. Termedia Publishing House 2011-06 2011-07-11 /pmc/articles/PMC3258754/ /pubmed/22295037 http://dx.doi.org/10.5114/aoms.2011.23421 Text en Copyright © 2011 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Research Jabłonowski, Zbigniew Reszka, Edyta Gromadzińska, Jolanta Wąsowicz, Wojciech Sosnowski, Marek Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title | Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title_full | Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title_fullStr | Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title_full_unstemmed | Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title_short | Hypermethylation of p16 and DAPK promoter gene regions in patients with non-invasive urinary bladder cancer |
title_sort | hypermethylation of p16 and dapk promoter gene regions in patients with non-invasive urinary bladder cancer |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258754/ https://www.ncbi.nlm.nih.gov/pubmed/22295037 http://dx.doi.org/10.5114/aoms.2011.23421 |
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