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A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice

INTRODUCTION: The aim of this preliminary study was to evaluate the effect of long-term progesterone (P4) treatment on structural and functional deficits associated with the hippocampus. MATERIAL AND METHODS: Mice served as sham controls or were bilaterally ovariectomized (Ovx), and a 90-day regimen...

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Autores principales: He, Lu, Yang, Hui, Zhai, Li-dong, Shao, Heng, Li, Yun-sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258758/
https://www.ncbi.nlm.nih.gov/pubmed/22295021
http://dx.doi.org/10.5114/aoms.2011.23402
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author He, Lu
Yang, Hui
Zhai, Li-dong
Shao, Heng
Li, Yun-sheng
author_facet He, Lu
Yang, Hui
Zhai, Li-dong
Shao, Heng
Li, Yun-sheng
author_sort He, Lu
collection PubMed
description INTRODUCTION: The aim of this preliminary study was to evaluate the effect of long-term progesterone (P4) treatment on structural and functional deficits associated with the hippocampus. MATERIAL AND METHODS: Mice served as sham controls or were bilaterally ovariectomized (Ovx), and a 90-day regimen of placebo or P4 was applied to the animal. After the administration, the acquisition and retrieval of mice in contextual fear conditioning (CFC) and a water maze were examined. Hippocampal tissues from some mice in each group were stained with cresyl violet, and the remainder were taken for determining the antioxidant power. RESULTS: Compared with placebo controls, the time spent on freezing was higher and the latencies were longer for mice given high-dose P4 (HP) (p < 0.05) in CFC, and the HP group also had longer searching time spent in the target quadrant (p < 0.05) in the water maze. Compared with placebo controls, the cell number of hippocampus CA1, CA3 and DG was significantly higher in the HP group (p < 0.05), and the thickness of the cell layer in CA1 and DG was also higher in the HP group (p < 0.05). All the oxidative stress biomarkers show that the antioxidative activity in hippocampus tissue from the HP and LP groups is higher than that in placebo controls (p < 0.05). CONCLUSIONS: Ovx impairs learning and memory of mice, which can be rescued by a long-term regimen of HP via its antioxidant effects.
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spelling pubmed-32587582012-01-31 A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice He, Lu Yang, Hui Zhai, Li-dong Shao, Heng Li, Yun-sheng Arch Med Sci Basic Research INTRODUCTION: The aim of this preliminary study was to evaluate the effect of long-term progesterone (P4) treatment on structural and functional deficits associated with the hippocampus. MATERIAL AND METHODS: Mice served as sham controls or were bilaterally ovariectomized (Ovx), and a 90-day regimen of placebo or P4 was applied to the animal. After the administration, the acquisition and retrieval of mice in contextual fear conditioning (CFC) and a water maze were examined. Hippocampal tissues from some mice in each group were stained with cresyl violet, and the remainder were taken for determining the antioxidant power. RESULTS: Compared with placebo controls, the time spent on freezing was higher and the latencies were longer for mice given high-dose P4 (HP) (p < 0.05) in CFC, and the HP group also had longer searching time spent in the target quadrant (p < 0.05) in the water maze. Compared with placebo controls, the cell number of hippocampus CA1, CA3 and DG was significantly higher in the HP group (p < 0.05), and the thickness of the cell layer in CA1 and DG was also higher in the HP group (p < 0.05). All the oxidative stress biomarkers show that the antioxidative activity in hippocampus tissue from the HP and LP groups is higher than that in placebo controls (p < 0.05). CONCLUSIONS: Ovx impairs learning and memory of mice, which can be rescued by a long-term regimen of HP via its antioxidant effects. Termedia Publishing House 2011-06 2011-07-11 /pmc/articles/PMC3258758/ /pubmed/22295021 http://dx.doi.org/10.5114/aoms.2011.23402 Text en Copyright © 2011 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
He, Lu
Yang, Hui
Zhai, Li-dong
Shao, Heng
Li, Yun-sheng
A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title_full A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title_fullStr A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title_full_unstemmed A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title_short A preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
title_sort preliminary study on progesterone antioxidation in promoting learning and memory of young ovariectomized mice
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258758/
https://www.ncbi.nlm.nih.gov/pubmed/22295021
http://dx.doi.org/10.5114/aoms.2011.23402
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