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Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease
INTRODUCTION: Toll-like receptors (TLRs) are an important link between innate and adaptive immunity. MATERIAL AND METHODS: Expression of TLR-2, TLR-4, and TLR-9 genes was assessed in 60 coronary artery disease (CAD) patients and 79 controls by SYBR Green 1 based real time PCR assay. RESULTS: Express...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258818/ https://www.ncbi.nlm.nih.gov/pubmed/22291822 http://dx.doi.org/10.5114/aoms.2011.25552 |
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author | Maitra, Arindam Shanker, Jayashree Dash, Debabrata Arvind, Prathima Kakkar, Vijay V. |
author_facet | Maitra, Arindam Shanker, Jayashree Dash, Debabrata Arvind, Prathima Kakkar, Vijay V. |
author_sort | Maitra, Arindam |
collection | PubMed |
description | INTRODUCTION: Toll-like receptors (TLRs) are an important link between innate and adaptive immunity. MATERIAL AND METHODS: Expression of TLR-2, TLR-4, and TLR-9 genes was assessed in 60 coronary artery disease (CAD) patients and 79 controls by SYBR Green 1 based real time PCR assay. RESULTS: Expression of the TLR-2 gene was found to be significantly elevated in cases (1.295 ±0.09) compared to the controls (1.033 ±0.08) (p = 0.015) whereas expression of the TLR-9 gene was significantly lower in cases (1.522 ±0.18) than in the controls (2.165 ±0.16) (p = 0.032). There was no difference in TLR-4 expression levels (p = 0.174). A significant correlation of TLR-2 was observed with TLR-4 (r = 0.803, p<0.0001) and TLR-9 (r = 0.264, p = 0.003) as well as between TLR-4 and TLR-9 (r = 0.303, p = 0.001). A significant association was seen between TLR 2 (OR 3.94, 95% CI 1.73-8.99, p = 0.001) and TLR-9 (OR 0.297, 95% CI 0.131-0.672, p = 0.004) with CAD after adjustment for age and gender. Statins did not affect TLR gene expression. CONCLUSIONS: The TLR-2, TLR-4 and TLR-9 genes exhibit a differential pattern of expression between CAD patients and controls in this Asian Indian cohort. This observation warrants further investigation, keeping in mind the infectious and inflammatory elements in perspective, in order to understand the true implications of TLR in the aetiopathology of CAD and consequent therapeutic implications. |
format | Online Article Text |
id | pubmed-3258818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-32588182012-01-30 Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease Maitra, Arindam Shanker, Jayashree Dash, Debabrata Arvind, Prathima Kakkar, Vijay V. Arch Med Sci Clinical Research INTRODUCTION: Toll-like receptors (TLRs) are an important link between innate and adaptive immunity. MATERIAL AND METHODS: Expression of TLR-2, TLR-4, and TLR-9 genes was assessed in 60 coronary artery disease (CAD) patients and 79 controls by SYBR Green 1 based real time PCR assay. RESULTS: Expression of the TLR-2 gene was found to be significantly elevated in cases (1.295 ±0.09) compared to the controls (1.033 ±0.08) (p = 0.015) whereas expression of the TLR-9 gene was significantly lower in cases (1.522 ±0.18) than in the controls (2.165 ±0.16) (p = 0.032). There was no difference in TLR-4 expression levels (p = 0.174). A significant correlation of TLR-2 was observed with TLR-4 (r = 0.803, p<0.0001) and TLR-9 (r = 0.264, p = 0.003) as well as between TLR-4 and TLR-9 (r = 0.303, p = 0.001). A significant association was seen between TLR 2 (OR 3.94, 95% CI 1.73-8.99, p = 0.001) and TLR-9 (OR 0.297, 95% CI 0.131-0.672, p = 0.004) with CAD after adjustment for age and gender. Statins did not affect TLR gene expression. CONCLUSIONS: The TLR-2, TLR-4 and TLR-9 genes exhibit a differential pattern of expression between CAD patients and controls in this Asian Indian cohort. This observation warrants further investigation, keeping in mind the infectious and inflammatory elements in perspective, in order to understand the true implications of TLR in the aetiopathology of CAD and consequent therapeutic implications. Termedia Publishing House 2011-11-08 2011-10 /pmc/articles/PMC3258818/ /pubmed/22291822 http://dx.doi.org/10.5114/aoms.2011.25552 Text en Copyright © 2011 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Research Maitra, Arindam Shanker, Jayashree Dash, Debabrata Arvind, Prathima Kakkar, Vijay V. Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title | Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title_full | Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title_fullStr | Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title_full_unstemmed | Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title_short | Understanding the expression of Toll-like receptors in Asian Indians predisposed to coronary artery disease |
title_sort | understanding the expression of toll-like receptors in asian indians predisposed to coronary artery disease |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258818/ https://www.ncbi.nlm.nih.gov/pubmed/22291822 http://dx.doi.org/10.5114/aoms.2011.25552 |
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