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Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines

Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using...

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Autores principales: Kishimoto, Satoko, Ishihara, Masayuki, Kanatani, Yasuhiro, Nambu, Masaki, Takikawa, Megumi, Sumi, Yuki, Nakamura, Shingo, Mori, Yasutaka, Hattori, Hidemi, Tanaka, Yoshihiro, Sato, Toshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258842/
https://www.ncbi.nlm.nih.gov/pubmed/22292106
http://dx.doi.org/10.1177/2041731411425419
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author Kishimoto, Satoko
Ishihara, Masayuki
Kanatani, Yasuhiro
Nambu, Masaki
Takikawa, Megumi
Sumi, Yuki
Nakamura, Shingo
Mori, Yasutaka
Hattori, Hidemi
Tanaka, Yoshihiro
Sato, Toshinori
author_facet Kishimoto, Satoko
Ishihara, Masayuki
Kanatani, Yasuhiro
Nambu, Masaki
Takikawa, Megumi
Sumi, Yuki
Nakamura, Shingo
Mori, Yasutaka
Hattori, Hidemi
Tanaka, Yoshihiro
Sato, Toshinori
author_sort Kishimoto, Satoko
collection PubMed
description Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium.
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spelling pubmed-32588422012-01-30 Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines Kishimoto, Satoko Ishihara, Masayuki Kanatani, Yasuhiro Nambu, Masaki Takikawa, Megumi Sumi, Yuki Nakamura, Shingo Mori, Yasutaka Hattori, Hidemi Tanaka, Yoshihiro Sato, Toshinori J Tissue Eng Article Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium. SAGE Publications 2011-10-30 /pmc/articles/PMC3258842/ /pubmed/22292106 http://dx.doi.org/10.1177/2041731411425419 Text en © The Author(s) 2011 http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kishimoto, Satoko
Ishihara, Masayuki
Kanatani, Yasuhiro
Nambu, Masaki
Takikawa, Megumi
Sumi, Yuki
Nakamura, Shingo
Mori, Yasutaka
Hattori, Hidemi
Tanaka, Yoshihiro
Sato, Toshinori
Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title_full Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title_fullStr Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title_full_unstemmed Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title_short Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines
title_sort selective expansion of cd34+ cells from mouse bone marrow cultured on lh/p mp-coated plates with adequate cytokines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258842/
https://www.ncbi.nlm.nih.gov/pubmed/22292106
http://dx.doi.org/10.1177/2041731411425419
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