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Sensing array of radically coupled genetic biopixels

While there has been significant progress in the development of engineering principles for synthetic biology, a substantial challenge is the construction of robust circuits in a noisy cellular environment. Such an environment leads to considerable intercellular variability in circuit behavior, which...

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Detalles Bibliográficos
Autores principales: Prindle, Arthur, Samayoa, Phillip, Razinkov, Ivan, Danino, Tal, Tsimring, Lev S., Hasty, Jeff
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3259005/
https://www.ncbi.nlm.nih.gov/pubmed/22178928
http://dx.doi.org/10.1038/nature10722
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author Prindle, Arthur
Samayoa, Phillip
Razinkov, Ivan
Danino, Tal
Tsimring, Lev S.
Hasty, Jeff
author_facet Prindle, Arthur
Samayoa, Phillip
Razinkov, Ivan
Danino, Tal
Tsimring, Lev S.
Hasty, Jeff
author_sort Prindle, Arthur
collection PubMed
description While there has been significant progress in the development of engineering principles for synthetic biology, a substantial challenge is the construction of robust circuits in a noisy cellular environment. Such an environment leads to considerable intercellular variability in circuit behavior, which can hinder functionality at the colony level. Here, we engineer the synchronization of thousands of oscillating colony “biopixels” over centimetre length scales through the use of synergistic intercellular coupling involving quorum sensing within a colony and gas-phase redox signaling between colonies. We use this platform to construct an LCD-like macroscopic clock that can be used to sense arsenic via modulation of the oscillatory period. Given the repertoire of sensing capabilities of bacteria such as E. coli, the ability to coordinate their behavior over large length scales sets the stage for the construction of low cost genetic biosensors that are capable of detecting heavy metals and pathogens in the field.
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spelling pubmed-32590052012-07-05 Sensing array of radically coupled genetic biopixels Prindle, Arthur Samayoa, Phillip Razinkov, Ivan Danino, Tal Tsimring, Lev S. Hasty, Jeff Nature Article While there has been significant progress in the development of engineering principles for synthetic biology, a substantial challenge is the construction of robust circuits in a noisy cellular environment. Such an environment leads to considerable intercellular variability in circuit behavior, which can hinder functionality at the colony level. Here, we engineer the synchronization of thousands of oscillating colony “biopixels” over centimetre length scales through the use of synergistic intercellular coupling involving quorum sensing within a colony and gas-phase redox signaling between colonies. We use this platform to construct an LCD-like macroscopic clock that can be used to sense arsenic via modulation of the oscillatory period. Given the repertoire of sensing capabilities of bacteria such as E. coli, the ability to coordinate their behavior over large length scales sets the stage for the construction of low cost genetic biosensors that are capable of detecting heavy metals and pathogens in the field. 2011-12-18 /pmc/articles/PMC3259005/ /pubmed/22178928 http://dx.doi.org/10.1038/nature10722 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Prindle, Arthur
Samayoa, Phillip
Razinkov, Ivan
Danino, Tal
Tsimring, Lev S.
Hasty, Jeff
Sensing array of radically coupled genetic biopixels
title Sensing array of radically coupled genetic biopixels
title_full Sensing array of radically coupled genetic biopixels
title_fullStr Sensing array of radically coupled genetic biopixels
title_full_unstemmed Sensing array of radically coupled genetic biopixels
title_short Sensing array of radically coupled genetic biopixels
title_sort sensing array of radically coupled genetic biopixels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3259005/
https://www.ncbi.nlm.nih.gov/pubmed/22178928
http://dx.doi.org/10.1038/nature10722
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