Reverse protection assay: a tool to analyze transcriptional rates from individual promoters

Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello r...

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Detalles Bibliográficos
Autores principales: Zubo, Yan O, Kusnetsov, Victor V, Börner, Thomas, Liere, Karsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3259058/
https://www.ncbi.nlm.nih.gov/pubmed/22185205
http://dx.doi.org/10.1186/1746-4811-7-47
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author Zubo, Yan O
Kusnetsov, Victor V
Börner, Thomas
Liere, Karsten
author_facet Zubo, Yan O
Kusnetsov, Victor V
Börner, Thomas
Liere, Karsten
author_sort Zubo, Yan O
collection PubMed
description Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
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spelling pubmed-32590582012-01-17 Reverse protection assay: a tool to analyze transcriptional rates from individual promoters Zubo, Yan O Kusnetsov, Victor V Börner, Thomas Liere, Karsten Plant Methods Methodology Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing. BioMed Central 2011-12-20 /pmc/articles/PMC3259058/ /pubmed/22185205 http://dx.doi.org/10.1186/1746-4811-7-47 Text en Copyright ©2011 Zubo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Zubo, Yan O
Kusnetsov, Victor V
Börner, Thomas
Liere, Karsten
Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title_full Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title_fullStr Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title_full_unstemmed Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title_short Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
title_sort reverse protection assay: a tool to analyze transcriptional rates from individual promoters
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3259058/
https://www.ncbi.nlm.nih.gov/pubmed/22185205
http://dx.doi.org/10.1186/1746-4811-7-47
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