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First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China
The increasing prevalence of insecticide resistance in Anopheles sinensis, a major vector of malaria in Jiangsu province in eastern China, threatens to compromise the successful use of insecticides in malaria control strategies. It is therefore vital to understand the insecticide resistance status o...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260143/ https://www.ncbi.nlm.nih.gov/pubmed/22272229 http://dx.doi.org/10.1371/journal.pone.0029242 |
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author | Tan, Wei-Long Wang, Zhong-Ming Li, Chun-Xiao Chu, Hong-Liang Xu, Yan Dong, Yan-De Wang, Zhong-can Chen, Dong-Ya Liu, Hui Liu, Da-Peng Liu, Nannan Sun, Jun Zhao, Tongyan |
author_facet | Tan, Wei-Long Wang, Zhong-Ming Li, Chun-Xiao Chu, Hong-Liang Xu, Yan Dong, Yan-De Wang, Zhong-can Chen, Dong-Ya Liu, Hui Liu, Da-Peng Liu, Nannan Sun, Jun Zhao, Tongyan |
author_sort | Tan, Wei-Long |
collection | PubMed |
description | The increasing prevalence of insecticide resistance in Anopheles sinensis, a major vector of malaria in Jiangsu province in eastern China, threatens to compromise the successful use of insecticides in malaria control strategies. It is therefore vital to understand the insecticide resistance status of An. sinensis in the region. This study examined the nucleotide diversity of the para-sodium channel and knockdown resistance (kdr) in five field populations of adult An. sinensis mosquitoes collected in Jiangsu province, identifying the L1014F and L1014C substitutions for the first time. Competitive polymerase chain reaction (PCR) amplification of specific allele (cPASA) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for resistance diagnosis were developed and validated. Comparing the results with direct sequencing revealed that the PCR-RFLP method was more sensitive and specific whereas the cPASA method was more convenient and suitable. The significant positive correlation between kdr allele frequency and bioassay-based resistance phenotype demonstrates that the frequency of L1014F and L1014C substitutions in the kdr gene provides a useful molecular marker for monitoring beta-cypermethrin resistance in natural populations of An. sinensis. Our results point to the L1014F substitution as the key mutation associated with beta-cypermethrin resistance. The high resistance and mutation frequency detected in the five populations also suggest cross-resistance with other pyrethroids may occur in An. sinensis, highlighting the need for further surveys to map insecticide resistance in China and the adoption of a rational management of insecticide application for resistance management and mosquito vector control. |
format | Online Article Text |
id | pubmed-3260143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32601432012-01-23 First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China Tan, Wei-Long Wang, Zhong-Ming Li, Chun-Xiao Chu, Hong-Liang Xu, Yan Dong, Yan-De Wang, Zhong-can Chen, Dong-Ya Liu, Hui Liu, Da-Peng Liu, Nannan Sun, Jun Zhao, Tongyan PLoS One Research Article The increasing prevalence of insecticide resistance in Anopheles sinensis, a major vector of malaria in Jiangsu province in eastern China, threatens to compromise the successful use of insecticides in malaria control strategies. It is therefore vital to understand the insecticide resistance status of An. sinensis in the region. This study examined the nucleotide diversity of the para-sodium channel and knockdown resistance (kdr) in five field populations of adult An. sinensis mosquitoes collected in Jiangsu province, identifying the L1014F and L1014C substitutions for the first time. Competitive polymerase chain reaction (PCR) amplification of specific allele (cPASA) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) for resistance diagnosis were developed and validated. Comparing the results with direct sequencing revealed that the PCR-RFLP method was more sensitive and specific whereas the cPASA method was more convenient and suitable. The significant positive correlation between kdr allele frequency and bioassay-based resistance phenotype demonstrates that the frequency of L1014F and L1014C substitutions in the kdr gene provides a useful molecular marker for monitoring beta-cypermethrin resistance in natural populations of An. sinensis. Our results point to the L1014F substitution as the key mutation associated with beta-cypermethrin resistance. The high resistance and mutation frequency detected in the five populations also suggest cross-resistance with other pyrethroids may occur in An. sinensis, highlighting the need for further surveys to map insecticide resistance in China and the adoption of a rational management of insecticide application for resistance management and mosquito vector control. Public Library of Science 2012-01-17 /pmc/articles/PMC3260143/ /pubmed/22272229 http://dx.doi.org/10.1371/journal.pone.0029242 Text en Tan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tan, Wei-Long Wang, Zhong-Ming Li, Chun-Xiao Chu, Hong-Liang Xu, Yan Dong, Yan-De Wang, Zhong-can Chen, Dong-Ya Liu, Hui Liu, Da-Peng Liu, Nannan Sun, Jun Zhao, Tongyan First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title | First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title_full | First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title_fullStr | First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title_full_unstemmed | First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title_short | First Report on Co-Occurrence Knockdown Resistance Mutations and Susceptibility to Beta-Cypermethrin in Anopheles sinensis from Jiangsu Province, China |
title_sort | first report on co-occurrence knockdown resistance mutations and susceptibility to beta-cypermethrin in anopheles sinensis from jiangsu province, china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260143/ https://www.ncbi.nlm.nih.gov/pubmed/22272229 http://dx.doi.org/10.1371/journal.pone.0029242 |
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