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Simultaneous Assessment of Asp Isomerization and Asn Deamidation in Recombinant Antibodies by LC-MS following Incubation at Elevated Temperatures

The degradation of proteins by asparagine deamidation and aspartate isomerization is one of several chemical degradation pathways for recombinant antibodies. In this study, we have identified two solvent accessible degradation sites (light chain aspartate-56 and heavy chain aspartate-99/101) in the...

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Detalles Bibliográficos
Autores principales:	Diepold, Katharina, Bomans, Katrin, Wiedmann, Michael, Zimmermann, Boris, Petzold, Andreas, Schlothauer, Tilman, Mueller, Robert, Moritz, Bernd, Stracke, Jan Olaf, Mølhøj, Michael, Reusch, Dietmar, Bulau, Patrick
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260267/ https://www.ncbi.nlm.nih.gov/pubmed/22272329 http://dx.doi.org/10.1371/journal.pone.0030295

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