The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach

A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid...

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Detalles Bibliográficos
Autores principales: Wang, Joanne H., Wang, Kevin, Bartling, Brandon, Liu, Chung-Chiun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260609/
https://www.ncbi.nlm.nih.gov/pubmed/22291532
http://dx.doi.org/10.3390/s91108709
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author Wang, Joanne H.
Wang, Kevin
Bartling, Brandon
Liu, Chung-Chiun
author_facet Wang, Joanne H.
Wang, Kevin
Bartling, Brandon
Liu, Chung-Chiun
author_sort Wang, Joanne H.
collection PubMed
description A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.
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spelling pubmed-32606092012-01-30 The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach Wang, Joanne H. Wang, Kevin Bartling, Brandon Liu, Chung-Chiun Sensors (Basel) Article A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding. Molecular Diversity Preservation International (MDPI) 2009-10-30 /pmc/articles/PMC3260609/ /pubmed/22291532 http://dx.doi.org/10.3390/s91108709 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Joanne H.
Wang, Kevin
Bartling, Brandon
Liu, Chung-Chiun
The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title_full The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title_fullStr The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title_full_unstemmed The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title_short The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
title_sort detection of alkaline phosphatase using an electrochemical biosensor in a single-step approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260609/
https://www.ncbi.nlm.nih.gov/pubmed/22291532
http://dx.doi.org/10.3390/s91108709
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