Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore

[Image: see text] Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,...

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Autores principales: Chalmers, Susan, Caldwell, Stuart T., Quin, Caroline, Prime, Tracy A., James, Andrew M., Cairns, Andrew G., Murphy, Michael P., McCarron, John G., Hartley, Richard C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260739/
https://www.ncbi.nlm.nih.gov/pubmed/22239373
http://dx.doi.org/10.1021/ja2077922
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author Chalmers, Susan
Caldwell, Stuart T.
Quin, Caroline
Prime, Tracy A.
James, Andrew M.
Cairns, Andrew G.
Murphy, Michael P.
McCarron, John G.
Hartley, Richard C.
author_facet Chalmers, Susan
Caldwell, Stuart T.
Quin, Caroline
Prime, Tracy A.
James, Andrew M.
Cairns, Andrew G.
Murphy, Michael P.
McCarron, John G.
Hartley, Richard C.
author_sort Chalmers, Susan
collection PubMed
description [Image: see text] Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.
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spelling pubmed-32607392012-01-19 Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore Chalmers, Susan Caldwell, Stuart T. Quin, Caroline Prime, Tracy A. James, Andrew M. Cairns, Andrew G. Murphy, Michael P. McCarron, John G. Hartley, Richard C. J Am Chem Soc [Image: see text] Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity. American Chemical Society 2011-12-22 2012-01-18 /pmc/articles/PMC3260739/ /pubmed/22239373 http://dx.doi.org/10.1021/ja2077922 Text en Copyright © 2011 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Chalmers, Susan
Caldwell, Stuart T.
Quin, Caroline
Prime, Tracy A.
James, Andrew M.
Cairns, Andrew G.
Murphy, Michael P.
McCarron, John G.
Hartley, Richard C.
Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title_full Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title_fullStr Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title_full_unstemmed Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title_short Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore
title_sort selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260739/
https://www.ncbi.nlm.nih.gov/pubmed/22239373
http://dx.doi.org/10.1021/ja2077922
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