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IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specifi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260819/ https://www.ncbi.nlm.nih.gov/pubmed/21399237 |
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author | He, Jian Wu, Jian Jiao, Yuchen Wagner-Johnston, Nina Ambinder, Richard F. Diaz, Luis A Kinzler, Kenneth W Vogelstein, Bert Papadopoulos, Nickolas |
author_facet | He, Jian Wu, Jian Jiao, Yuchen Wagner-Johnston, Nina Ambinder, Richard F. Diaz, Luis A Kinzler, Kenneth W Vogelstein, Bert Papadopoulos, Nickolas |
author_sort | He, Jian |
collection | PubMed |
description | New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specific rearranged DNA fragments in patients' plasma. By capturing and sequencing the IgH genomic regions (IgCap), we were able to detect and precisely determine the sequence of rearranged IgH loci in the tumors of three NHL patients. Moreover, circulating rearranged DNA fragments could be identified in the plasma of all three patients. Even in cases wherein tumor biopsies were unavailable, we were able to use the IgH capture approach to identify rearranged DNA loci in the plasma of 8 of 14 patients. IgCap may enable a more informed management of selected patients with NHL and other B-cell malignancies in the future. |
format | Online Article Text |
id | pubmed-3260819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-32608192012-01-18 IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients He, Jian Wu, Jian Jiao, Yuchen Wagner-Johnston, Nina Ambinder, Richard F. Diaz, Luis A Kinzler, Kenneth W Vogelstein, Bert Papadopoulos, Nickolas Oncotarget Research Papers New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specific rearranged DNA fragments in patients' plasma. By capturing and sequencing the IgH genomic regions (IgCap), we were able to detect and precisely determine the sequence of rearranged IgH loci in the tumors of three NHL patients. Moreover, circulating rearranged DNA fragments could be identified in the plasma of all three patients. Even in cases wherein tumor biopsies were unavailable, we were able to use the IgH capture approach to identify rearranged DNA loci in the plasma of 8 of 14 patients. IgCap may enable a more informed management of selected patients with NHL and other B-cell malignancies in the future. Impact Journals LLC 2011-03-08 /pmc/articles/PMC3260819/ /pubmed/21399237 Text en Copyright: © 2011 He et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Papers He, Jian Wu, Jian Jiao, Yuchen Wagner-Johnston, Nina Ambinder, Richard F. Diaz, Luis A Kinzler, Kenneth W Vogelstein, Bert Papadopoulos, Nickolas IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title | IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title_full | IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title_fullStr | IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title_full_unstemmed | IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title_short | IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients |
title_sort | igh gene rearrangements as plasma biomarkers in non-hodgkin's lymphoma patients |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260819/ https://www.ncbi.nlm.nih.gov/pubmed/21399237 |
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