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IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients

New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specifi...

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Autores principales: He, Jian, Wu, Jian, Jiao, Yuchen, Wagner-Johnston, Nina, Ambinder, Richard F., Diaz, Luis A, Kinzler, Kenneth W, Vogelstein, Bert, Papadopoulos, Nickolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260819/
https://www.ncbi.nlm.nih.gov/pubmed/21399237
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author He, Jian
Wu, Jian
Jiao, Yuchen
Wagner-Johnston, Nina
Ambinder, Richard F.
Diaz, Luis A
Kinzler, Kenneth W
Vogelstein, Bert
Papadopoulos, Nickolas
author_facet He, Jian
Wu, Jian
Jiao, Yuchen
Wagner-Johnston, Nina
Ambinder, Richard F.
Diaz, Luis A
Kinzler, Kenneth W
Vogelstein, Bert
Papadopoulos, Nickolas
author_sort He, Jian
collection PubMed
description New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specific rearranged DNA fragments in patients' plasma. By capturing and sequencing the IgH genomic regions (IgCap), we were able to detect and precisely determine the sequence of rearranged IgH loci in the tumors of three NHL patients. Moreover, circulating rearranged DNA fragments could be identified in the plasma of all three patients. Even in cases wherein tumor biopsies were unavailable, we were able to use the IgH capture approach to identify rearranged DNA loci in the plasma of 8 of 14 patients. IgCap may enable a more informed management of selected patients with NHL and other B-cell malignancies in the future.
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spelling pubmed-32608192012-01-18 IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients He, Jian Wu, Jian Jiao, Yuchen Wagner-Johnston, Nina Ambinder, Richard F. Diaz, Luis A Kinzler, Kenneth W Vogelstein, Bert Papadopoulos, Nickolas Oncotarget Research Papers New biomarkers with improved accuracy could be helpful for monitoring disease in patients with Non-Hodgkin's lymphomas (NHL). Towards this end, we have explored the feasibility of identifying the sequence of rearranged IgH genes using next-generation sequencing, then using PCR to detect specific rearranged DNA fragments in patients' plasma. By capturing and sequencing the IgH genomic regions (IgCap), we were able to detect and precisely determine the sequence of rearranged IgH loci in the tumors of three NHL patients. Moreover, circulating rearranged DNA fragments could be identified in the plasma of all three patients. Even in cases wherein tumor biopsies were unavailable, we were able to use the IgH capture approach to identify rearranged DNA loci in the plasma of 8 of 14 patients. IgCap may enable a more informed management of selected patients with NHL and other B-cell malignancies in the future. Impact Journals LLC 2011-03-08 /pmc/articles/PMC3260819/ /pubmed/21399237 Text en Copyright: © 2011 He et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Papers
He, Jian
Wu, Jian
Jiao, Yuchen
Wagner-Johnston, Nina
Ambinder, Richard F.
Diaz, Luis A
Kinzler, Kenneth W
Vogelstein, Bert
Papadopoulos, Nickolas
IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title_full IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title_fullStr IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title_full_unstemmed IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title_short IgH gene rearrangements as plasma biomarkers in Non-Hodgkin's Lymphoma patients
title_sort igh gene rearrangements as plasma biomarkers in non-hodgkin's lymphoma patients
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260819/
https://www.ncbi.nlm.nih.gov/pubmed/21399237
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