Simultaneous determination of geniposide, chlorogenic acid, crocin1, and rutin in crude and processed Fructus Gardeniae extracts by high performance liquid chromatography

BACKGROUND: Fructus Gardeniae, commonly used traditional Chinese medicine (TCM) called Zhizi in chinese, is derived from the dried fruit of Gardenia jasminoides Ellis of the Madder Family. To our knowledge, previously reported analytical methods were not developing for simultaneous determination of geniposide, chlorogenic acid, crocin1, and rutin in Fructus Gardeniae and its processed products of chaozheng pin (CZP) extracts. MATERIALS AND METHODS: In this study, a HPLC method was developed for simultaneous determination four major active components in Fructus Gardeniae and its processed products. RESULTS: The contents of geniposide, chlorogenic acid, crocin1, and rutin in Fructus Gardeniae samples analyzed were 2.492 - 4.242%, 0.162 - 0.407%, 0.417 -0.837%, and 0.116 - 0.251%, respectively. CONCLUSION: The developed method can be applied to the intrinsic quality control of Fructus Gardeniae.