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Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system

In the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type III secretion system (TTSS) in 64 Aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. We observed that contact of the bacteria with host epithelial cells is a prere...

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Autores principales: Krzymińska, Sylwia, Mokracka, Joanna, Koczura, Ryszard, Ćwiertnia, Anna, Kaznowski, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261397/
https://www.ncbi.nlm.nih.gov/pubmed/21809027
http://dx.doi.org/10.1007/s10482-011-9627-5
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author Krzymińska, Sylwia
Mokracka, Joanna
Koczura, Ryszard
Ćwiertnia, Anna
Kaznowski, Adam
author_facet Krzymińska, Sylwia
Mokracka, Joanna
Koczura, Ryszard
Ćwiertnia, Anna
Kaznowski, Adam
author_sort Krzymińska, Sylwia
collection PubMed
description In the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type III secretion system (TTSS) in 64 Aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. We observed that contact of the bacteria with host epithelial cells is a prerequisite for their cytotoxicity at 3 h incubation. Cell-contact cytotoxic activity of the strains was strongly associated with the presence of the TTSS. Culture supernatants of the strains induced low cytotoxicity effects at the same time of incubation. Cell-free supernatants of 61 (95%) isolates expressed cytotoxic activity which caused the destruction of HEp-2 cells at 24 h. Moreover, 44% strains were cytotonic towards CHO cells and 46% of strains invaded epithelial cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-011-9627-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-32613972012-02-03 Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system Krzymińska, Sylwia Mokracka, Joanna Koczura, Ryszard Ćwiertnia, Anna Kaznowski, Adam Antonie Van Leeuwenhoek Original Paper In the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type III secretion system (TTSS) in 64 Aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. We observed that contact of the bacteria with host epithelial cells is a prerequisite for their cytotoxicity at 3 h incubation. Cell-contact cytotoxic activity of the strains was strongly associated with the presence of the TTSS. Culture supernatants of the strains induced low cytotoxicity effects at the same time of incubation. Cell-free supernatants of 61 (95%) isolates expressed cytotoxic activity which caused the destruction of HEp-2 cells at 24 h. Moreover, 44% strains were cytotonic towards CHO cells and 46% of strains invaded epithelial cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-011-9627-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2011-08-02 2012 /pmc/articles/PMC3261397/ /pubmed/21809027 http://dx.doi.org/10.1007/s10482-011-9627-5 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Krzymińska, Sylwia
Mokracka, Joanna
Koczura, Ryszard
Ćwiertnia, Anna
Kaznowski, Adam
Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title_full Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title_fullStr Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title_full_unstemmed Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title_short Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system
title_sort aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type iii secretion system
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261397/
https://www.ncbi.nlm.nih.gov/pubmed/21809027
http://dx.doi.org/10.1007/s10482-011-9627-5
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