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A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes
We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid isolation of plasma membranes (PMs), enabling high-resolution proteomic, glycomic and lipidomic analyses of the cell surface. We used this technology to characterize the effects of presenilin knockout...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261717/ https://www.ncbi.nlm.nih.gov/pubmed/22027552 http://dx.doi.org/10.1038/msb.2011.74 |
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author | Thimiri Govinda Raj, Deepak B Ghesquière, Bart Tharkeshwar, Arun Kumar Coen, Katrijn Derua, Rita Vanderschaeghe, Dieter Rysman, Evelien Bagadi, Murali Baatsen, Pieter De Strooper, Bart Waelkens, Etienne Borghs, Gustaaf Callewaert, Nico Swinnen, Johan Gevaert, Kris Annaert, Wim |
author_facet | Thimiri Govinda Raj, Deepak B Ghesquière, Bart Tharkeshwar, Arun Kumar Coen, Katrijn Derua, Rita Vanderschaeghe, Dieter Rysman, Evelien Bagadi, Murali Baatsen, Pieter De Strooper, Bart Waelkens, Etienne Borghs, Gustaaf Callewaert, Nico Swinnen, Johan Gevaert, Kris Annaert, Wim |
author_sort | Thimiri Govinda Raj, Deepak B |
collection | PubMed |
description | We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid isolation of plasma membranes (PMs), enabling high-resolution proteomic, glycomic and lipidomic analyses of the cell surface. We used this technology to characterize the effects of presenilin knockout on the PM composition of mouse embryonic fibroblasts. We found that many proteins are selectively downregulated at the cell surface of presenilin knockout cells concomitant with lowered surface levels of cholesterol and certain sphingomyelin species, indicating defects in specific endosomal transport routes to and/or from the cell surface. Snapshots of N-glycoproteomics and cell surface glycan profiling further underscored the power and versatility of this novel methodology. Since PM proteins provide many pathologically relevant biomarkers representing two-thirds of the currently used drug targets, this novel technology has great potential for biomedical and pharmaceutical applications. |
format | Online Article Text |
id | pubmed-3261717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-32617172012-01-20 A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes Thimiri Govinda Raj, Deepak B Ghesquière, Bart Tharkeshwar, Arun Kumar Coen, Katrijn Derua, Rita Vanderschaeghe, Dieter Rysman, Evelien Bagadi, Murali Baatsen, Pieter De Strooper, Bart Waelkens, Etienne Borghs, Gustaaf Callewaert, Nico Swinnen, Johan Gevaert, Kris Annaert, Wim Mol Syst Biol Article We manufactured a novel type of lipid-coated superparamagnetic nanoparticles that allow for a rapid isolation of plasma membranes (PMs), enabling high-resolution proteomic, glycomic and lipidomic analyses of the cell surface. We used this technology to characterize the effects of presenilin knockout on the PM composition of mouse embryonic fibroblasts. We found that many proteins are selectively downregulated at the cell surface of presenilin knockout cells concomitant with lowered surface levels of cholesterol and certain sphingomyelin species, indicating defects in specific endosomal transport routes to and/or from the cell surface. Snapshots of N-glycoproteomics and cell surface glycan profiling further underscored the power and versatility of this novel methodology. Since PM proteins provide many pathologically relevant biomarkers representing two-thirds of the currently used drug targets, this novel technology has great potential for biomedical and pharmaceutical applications. European Molecular Biology Organization 2011-10-25 /pmc/articles/PMC3261717/ /pubmed/22027552 http://dx.doi.org/10.1038/msb.2011.74 Text en Copyright © 2011, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Article Thimiri Govinda Raj, Deepak B Ghesquière, Bart Tharkeshwar, Arun Kumar Coen, Katrijn Derua, Rita Vanderschaeghe, Dieter Rysman, Evelien Bagadi, Murali Baatsen, Pieter De Strooper, Bart Waelkens, Etienne Borghs, Gustaaf Callewaert, Nico Swinnen, Johan Gevaert, Kris Annaert, Wim A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title | A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title_full | A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title_fullStr | A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title_full_unstemmed | A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title_short | A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
title_sort | novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261717/ https://www.ncbi.nlm.nih.gov/pubmed/22027552 http://dx.doi.org/10.1038/msb.2011.74 |
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