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Prolonged morphine administration alters protein expression in the rat myocardium
BACKGROUND: Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alter...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261841/ https://www.ncbi.nlm.nih.gov/pubmed/22129148 http://dx.doi.org/10.1186/1423-0127-18-89 |
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author | Drastichova, Zdenka Skrabalova, Jitka Neckar, Jan Kolar, Frantisek Novotny, Jiri |
author_facet | Drastichova, Zdenka Skrabalova, Jitka Neckar, Jan Kolar, Frantisek Novotny, Jiri |
author_sort | Drastichova, Zdenka |
collection | PubMed |
description | BACKGROUND: Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in rat myocardial protein levels after prolonged morphine treatment. METHODS: Morphine was administered to adult male Wistar rats in high doses (10 mg/kg per day) for 10 days. Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins. RESULTS: Nine proteins were found to show markedly altered expression levels in samples from morphine-treaded rats and these proteins were identified by mass spectrometric analysis. They belong to different cell pathways including signaling, cytoprotective, and structural elements. CONCLUSIONS: The present identification of several important myocardial proteins altered by prolonged morphine treatment points to global effects of this drug on heart tissue. These findings represent an initial step toward a more complex view on the action of morphine on the heart. |
format | Online Article Text |
id | pubmed-3261841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32618412012-01-20 Prolonged morphine administration alters protein expression in the rat myocardium Drastichova, Zdenka Skrabalova, Jitka Neckar, Jan Kolar, Frantisek Novotny, Jiri J Biomed Sci Research BACKGROUND: Morphine is used in clinical practice as a highly effective painkiller as well as the drug of choice for treatment of certain heart diseases. However, there is lack of information about its effect on protein expression in the heart. Therefore, here we aimed to identify the presumed alterations in rat myocardial protein levels after prolonged morphine treatment. METHODS: Morphine was administered to adult male Wistar rats in high doses (10 mg/kg per day) for 10 days. Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins. RESULTS: Nine proteins were found to show markedly altered expression levels in samples from morphine-treaded rats and these proteins were identified by mass spectrometric analysis. They belong to different cell pathways including signaling, cytoprotective, and structural elements. CONCLUSIONS: The present identification of several important myocardial proteins altered by prolonged morphine treatment points to global effects of this drug on heart tissue. These findings represent an initial step toward a more complex view on the action of morphine on the heart. BioMed Central 2011-11-30 /pmc/articles/PMC3261841/ /pubmed/22129148 http://dx.doi.org/10.1186/1423-0127-18-89 Text en Copyright ©2011 Drastichova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Drastichova, Zdenka Skrabalova, Jitka Neckar, Jan Kolar, Frantisek Novotny, Jiri Prolonged morphine administration alters protein expression in the rat myocardium |
title | Prolonged morphine administration alters protein expression in the rat myocardium |
title_full | Prolonged morphine administration alters protein expression in the rat myocardium |
title_fullStr | Prolonged morphine administration alters protein expression in the rat myocardium |
title_full_unstemmed | Prolonged morphine administration alters protein expression in the rat myocardium |
title_short | Prolonged morphine administration alters protein expression in the rat myocardium |
title_sort | prolonged morphine administration alters protein expression in the rat myocardium |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261841/ https://www.ncbi.nlm.nih.gov/pubmed/22129148 http://dx.doi.org/10.1186/1423-0127-18-89 |
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