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Accumulation of Endogenous LITAF in Aggresomes

LITAF is a 161 amino acid cellular protein which includes a proline rich N-terminus and a conserved C-terminal domain known as the simple-like domain. Mutations in LITAF have been identified in Charcot-Marie tooth disease, a disease characterized by protein aggregates. Cells transfected with cellula...

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Autores principales: Eaton, Heather E., Metcalf, Julie, Lacerda, Andressa Ferreira, Brunetti, Craig R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261850/
https://www.ncbi.nlm.nih.gov/pubmed/22276139
http://dx.doi.org/10.1371/journal.pone.0030003
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author Eaton, Heather E.
Metcalf, Julie
Lacerda, Andressa Ferreira
Brunetti, Craig R.
author_facet Eaton, Heather E.
Metcalf, Julie
Lacerda, Andressa Ferreira
Brunetti, Craig R.
author_sort Eaton, Heather E.
collection PubMed
description LITAF is a 161 amino acid cellular protein which includes a proline rich N-terminus and a conserved C-terminal domain known as the simple-like domain. Mutations in LITAF have been identified in Charcot-Marie tooth disease, a disease characterized by protein aggregates. Cells transfected with cellular LITAF reveal that LITAF is localized to late endosomes/lysosomes. Here we investigated the intracellular localization of endogenous LITAF. We demonstrated that endogenous LITAF accumulates at a discrete cytoplasmic site in BGMK cells that we identify as the aggresome. To determine the domain within LITAF that is responsible for the localization of LITAF to aggresomes, we created a construct that contained the C-terminal simple-like domain of LITAF and found that this construct also localizes to aggresomes. These data suggest the simple-like domain is responsible for targeting endogenous LITAF to the aggresome.
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spelling pubmed-32618502012-01-24 Accumulation of Endogenous LITAF in Aggresomes Eaton, Heather E. Metcalf, Julie Lacerda, Andressa Ferreira Brunetti, Craig R. PLoS One Research Article LITAF is a 161 amino acid cellular protein which includes a proline rich N-terminus and a conserved C-terminal domain known as the simple-like domain. Mutations in LITAF have been identified in Charcot-Marie tooth disease, a disease characterized by protein aggregates. Cells transfected with cellular LITAF reveal that LITAF is localized to late endosomes/lysosomes. Here we investigated the intracellular localization of endogenous LITAF. We demonstrated that endogenous LITAF accumulates at a discrete cytoplasmic site in BGMK cells that we identify as the aggresome. To determine the domain within LITAF that is responsible for the localization of LITAF to aggresomes, we created a construct that contained the C-terminal simple-like domain of LITAF and found that this construct also localizes to aggresomes. These data suggest the simple-like domain is responsible for targeting endogenous LITAF to the aggresome. Public Library of Science 2012-01-19 /pmc/articles/PMC3261850/ /pubmed/22276139 http://dx.doi.org/10.1371/journal.pone.0030003 Text en Eaton et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Eaton, Heather E.
Metcalf, Julie
Lacerda, Andressa Ferreira
Brunetti, Craig R.
Accumulation of Endogenous LITAF in Aggresomes
title Accumulation of Endogenous LITAF in Aggresomes
title_full Accumulation of Endogenous LITAF in Aggresomes
title_fullStr Accumulation of Endogenous LITAF in Aggresomes
title_full_unstemmed Accumulation of Endogenous LITAF in Aggresomes
title_short Accumulation of Endogenous LITAF in Aggresomes
title_sort accumulation of endogenous litaf in aggresomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261850/
https://www.ncbi.nlm.nih.gov/pubmed/22276139
http://dx.doi.org/10.1371/journal.pone.0030003
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