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Expression of delta-catenin is associated with progression of human astrocytoma

BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its f...

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Autores principales: MingHao, Wang, Qianze, Dong, Di, Zhang, YunJie, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262777/
https://www.ncbi.nlm.nih.gov/pubmed/22151302
http://dx.doi.org/10.1186/1471-2407-11-514
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author MingHao, Wang
Qianze, Dong
Di, Zhang
YunJie, Wang
author_facet MingHao, Wang
Qianze, Dong
Di, Zhang
YunJie, Wang
author_sort MingHao, Wang
collection PubMed
description BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques. RESULTS: δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); p < 0.01). In addition, CTNND2 overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity. CONCLUSION: The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells.
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spelling pubmed-32627772012-01-21 Expression of delta-catenin is associated with progression of human astrocytoma MingHao, Wang Qianze, Dong Di, Zhang YunJie, Wang BMC Cancer Research Article BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques. RESULTS: δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); p < 0.01). In addition, CTNND2 overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity. CONCLUSION: The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells. BioMed Central 2011-12-12 /pmc/articles/PMC3262777/ /pubmed/22151302 http://dx.doi.org/10.1186/1471-2407-11-514 Text en Copyright ©2011 MingHao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
MingHao, Wang
Qianze, Dong
Di, Zhang
YunJie, Wang
Expression of delta-catenin is associated with progression of human astrocytoma
title Expression of delta-catenin is associated with progression of human astrocytoma
title_full Expression of delta-catenin is associated with progression of human astrocytoma
title_fullStr Expression of delta-catenin is associated with progression of human astrocytoma
title_full_unstemmed Expression of delta-catenin is associated with progression of human astrocytoma
title_short Expression of delta-catenin is associated with progression of human astrocytoma
title_sort expression of delta-catenin is associated with progression of human astrocytoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262777/
https://www.ncbi.nlm.nih.gov/pubmed/22151302
http://dx.doi.org/10.1186/1471-2407-11-514
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