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Expression of delta-catenin is associated with progression of human astrocytoma
BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262777/ https://www.ncbi.nlm.nih.gov/pubmed/22151302 http://dx.doi.org/10.1186/1471-2407-11-514 |
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author | MingHao, Wang Qianze, Dong Di, Zhang YunJie, Wang |
author_facet | MingHao, Wang Qianze, Dong Di, Zhang YunJie, Wang |
author_sort | MingHao, Wang |
collection | PubMed |
description | BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques. RESULTS: δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); p < 0.01). In addition, CTNND2 overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity. CONCLUSION: The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells. |
format | Online Article Text |
id | pubmed-3262777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32627772012-01-21 Expression of delta-catenin is associated with progression of human astrocytoma MingHao, Wang Qianze, Dong Di, Zhang YunJie, Wang BMC Cancer Research Article BACKGROUND: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. METHODS: We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques. RESULTS: δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); p < 0.01). In addition, CTNND2 overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity. CONCLUSION: The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells. BioMed Central 2011-12-12 /pmc/articles/PMC3262777/ /pubmed/22151302 http://dx.doi.org/10.1186/1471-2407-11-514 Text en Copyright ©2011 MingHao et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article MingHao, Wang Qianze, Dong Di, Zhang YunJie, Wang Expression of delta-catenin is associated with progression of human astrocytoma |
title | Expression of delta-catenin is associated with progression of human astrocytoma |
title_full | Expression of delta-catenin is associated with progression of human astrocytoma |
title_fullStr | Expression of delta-catenin is associated with progression of human astrocytoma |
title_full_unstemmed | Expression of delta-catenin is associated with progression of human astrocytoma |
title_short | Expression of delta-catenin is associated with progression of human astrocytoma |
title_sort | expression of delta-catenin is associated with progression of human astrocytoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262777/ https://www.ncbi.nlm.nih.gov/pubmed/22151302 http://dx.doi.org/10.1186/1471-2407-11-514 |
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