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Oxidative and antioxidative defense system in testicular torsion/detorsion

AIM: The present study was aimed to assess the early effects of ischemia/reperfusion injury on the oxidants and anti-oxidant defense status in rat testicular tissue by measuring MDA, glucose-6-phosphte dehydrogenase activity and reduced glutathione levels in a designated time frame sequel to reperfu...

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Autores principales: Elshaari, F. A., Elfagih, R. I., Sheriff, D S, Barassi, I. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263215/
https://www.ncbi.nlm.nih.gov/pubmed/22279313
http://dx.doi.org/10.4103/0970-1591.91436
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author Elshaari, F. A.
Elfagih, R. I.
Sheriff, D S
Barassi, I. F.
author_facet Elshaari, F. A.
Elfagih, R. I.
Sheriff, D S
Barassi, I. F.
author_sort Elshaari, F. A.
collection PubMed
description AIM: The present study was aimed to assess the early effects of ischemia/reperfusion injury on the oxidants and anti-oxidant defense status in rat testicular tissue by measuring MDA, glucose-6-phosphte dehydrogenase activity and reduced glutathione levels in a designated time frame sequel to reperfusion. Animals were divided randomly into six groups (12 animals per group) in the following order: Group I: Sham-operated control group (Cso) without the application of the torsion. Group 2: Torsion-induced ischemia group (T30 m): Ischemia was induced through the torsion of spermatic cord for a period of 30 min. Group 3: One hour reperfusion group after detorsion (T30 mR1 h). Group 4: Twenty-four hour reperfusion group after detorsion (T30 mR24 h). Group 5: Forty-eight hours reperfusion group after detorsion (T30mR48h). Group 6: One week reperfusion group after detorsion (T30mR1wk). RESULTS AND DISCUSSION: The oxidant-antioxidant system of the testicular tissue is altered during torsion as well as detorsion which results in the altered activities involved in the key enzyme of hexose monophosphate shunt pathway, glucose 6 phosphate dehydrogenase activity along with a reduction of glutathione (G.SH) content. The increase in G6PDH activity during torsion and followed by an increase in detorsion indicates the tissue's response to counter the oxidant stress caused by reduced blood supply. Continued exposure to such oxidant stressed physiological state of a tissue may lead to decreased capacity of the tissue to perform its physiological function such as testicular steroidogenesis and spermiogenesis shown in the present study.
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spelling pubmed-32632152012-01-25 Oxidative and antioxidative defense system in testicular torsion/detorsion Elshaari, F. A. Elfagih, R. I. Sheriff, D S Barassi, I. F. Indian J Urol Original Article AIM: The present study was aimed to assess the early effects of ischemia/reperfusion injury on the oxidants and anti-oxidant defense status in rat testicular tissue by measuring MDA, glucose-6-phosphte dehydrogenase activity and reduced glutathione levels in a designated time frame sequel to reperfusion. Animals were divided randomly into six groups (12 animals per group) in the following order: Group I: Sham-operated control group (Cso) without the application of the torsion. Group 2: Torsion-induced ischemia group (T30 m): Ischemia was induced through the torsion of spermatic cord for a period of 30 min. Group 3: One hour reperfusion group after detorsion (T30 mR1 h). Group 4: Twenty-four hour reperfusion group after detorsion (T30 mR24 h). Group 5: Forty-eight hours reperfusion group after detorsion (T30mR48h). Group 6: One week reperfusion group after detorsion (T30mR1wk). RESULTS AND DISCUSSION: The oxidant-antioxidant system of the testicular tissue is altered during torsion as well as detorsion which results in the altered activities involved in the key enzyme of hexose monophosphate shunt pathway, glucose 6 phosphate dehydrogenase activity along with a reduction of glutathione (G.SH) content. The increase in G6PDH activity during torsion and followed by an increase in detorsion indicates the tissue's response to counter the oxidant stress caused by reduced blood supply. Continued exposure to such oxidant stressed physiological state of a tissue may lead to decreased capacity of the tissue to perform its physiological function such as testicular steroidogenesis and spermiogenesis shown in the present study. Medknow Publications & Media Pvt Ltd 2011 /pmc/articles/PMC3263215/ /pubmed/22279313 http://dx.doi.org/10.4103/0970-1591.91436 Text en Copyright: © Indian Journal of Urology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Elshaari, F. A.
Elfagih, R. I.
Sheriff, D S
Barassi, I. F.
Oxidative and antioxidative defense system in testicular torsion/detorsion
title Oxidative and antioxidative defense system in testicular torsion/detorsion
title_full Oxidative and antioxidative defense system in testicular torsion/detorsion
title_fullStr Oxidative and antioxidative defense system in testicular torsion/detorsion
title_full_unstemmed Oxidative and antioxidative defense system in testicular torsion/detorsion
title_short Oxidative and antioxidative defense system in testicular torsion/detorsion
title_sort oxidative and antioxidative defense system in testicular torsion/detorsion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263215/
https://www.ncbi.nlm.nih.gov/pubmed/22279313
http://dx.doi.org/10.4103/0970-1591.91436
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AT barassiif oxidativeandantioxidativedefensesystemintesticulartorsiondetorsion