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Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264455/ https://www.ncbi.nlm.nih.gov/pubmed/22294903 http://dx.doi.org/10.3390/s100301846 |
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author | Kaewphinit, Thongchai Santiwatanakul, Somchai Promptmas, Chamras Chansiri, Kosum |
author_facet | Kaewphinit, Thongchai Santiwatanakul, Somchai Promptmas, Chamras Chansiri, Kosum |
author_sort | Kaewphinit, Thongchai |
collection | PubMed |
description | Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. |
format | Online Article Text |
id | pubmed-3264455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-32644552012-01-31 Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors Kaewphinit, Thongchai Santiwatanakul, Somchai Promptmas, Chamras Chansiri, Kosum Sensors (Basel) Article Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. Molecular Diversity Preservation International (MDPI) 2010-03-09 /pmc/articles/PMC3264455/ /pubmed/22294903 http://dx.doi.org/10.3390/s100301846 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kaewphinit, Thongchai Santiwatanakul, Somchai Promptmas, Chamras Chansiri, Kosum Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title | Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title_full | Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title_fullStr | Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title_full_unstemmed | Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title_short | Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors |
title_sort | detection of non-amplified mycobacterium tuberculosis genomic dna using piezoelectric dna-based biosensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264455/ https://www.ncbi.nlm.nih.gov/pubmed/22294903 http://dx.doi.org/10.3390/s100301846 |
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