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Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this s...

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Detalles Bibliográficos
Autores principales: Kaewphinit, Thongchai, Santiwatanakul, Somchai, Promptmas, Chamras, Chansiri, Kosum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264455/
https://www.ncbi.nlm.nih.gov/pubmed/22294903
http://dx.doi.org/10.3390/s100301846
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author Kaewphinit, Thongchai
Santiwatanakul, Somchai
Promptmas, Chamras
Chansiri, Kosum
author_facet Kaewphinit, Thongchai
Santiwatanakul, Somchai
Promptmas, Chamras
Chansiri, Kosum
author_sort Kaewphinit, Thongchai
collection PubMed
description Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.
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spelling pubmed-32644552012-01-31 Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors Kaewphinit, Thongchai Santiwatanakul, Somchai Promptmas, Chamras Chansiri, Kosum Sensors (Basel) Article Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. Molecular Diversity Preservation International (MDPI) 2010-03-09 /pmc/articles/PMC3264455/ /pubmed/22294903 http://dx.doi.org/10.3390/s100301846 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kaewphinit, Thongchai
Santiwatanakul, Somchai
Promptmas, Chamras
Chansiri, Kosum
Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title_full Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title_fullStr Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title_full_unstemmed Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title_short Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors
title_sort detection of non-amplified mycobacterium tuberculosis genomic dna using piezoelectric dna-based biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264455/
https://www.ncbi.nlm.nih.gov/pubmed/22294903
http://dx.doi.org/10.3390/s100301846
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