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Polar Electrophoresis: Shape of Two-Dimensional Maps Is as Important as Size

The performance of two-dimensional electrophoresis in conventional gels in Cartesian coordinates (2-DE) vs. polar coordinates (2-PE) is here evaluated. Although 2-DE is performed in much longer Immobiline gels in the first dimension (17 cm) vs. barely 7-cm in 2-PE, an equivalent resolving power is f...

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Detalles Bibliográficos
Autores principales: Millioni, Renato, Polati, Rita, Menini, Michele, Puricelli, Lucia, Miuzzo, Manuela, Tessari, Paolo, Novelli, Enrico, Righetti, Pier Giorgio, Cecconi, Daniela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264644/
https://www.ncbi.nlm.nih.gov/pubmed/22292075
http://dx.doi.org/10.1371/journal.pone.0030911