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Oncolytic Viruses: The Power of Directed Evolution
Attempts at developing oncolytic viruses have been primarily based on rational design. However, this approach has been met with limited success. An alternative approach employs directed evolution as a means of producing highly selective and potent anticancer viruses. In this method, diverse viruses...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265225/ https://www.ncbi.nlm.nih.gov/pubmed/22312363 http://dx.doi.org/10.1155/2012/586389 |
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author | Bauzon, Maxine Hermiston, Terry W. |
author_facet | Bauzon, Maxine Hermiston, Terry W. |
author_sort | Bauzon, Maxine |
collection | PubMed |
description | Attempts at developing oncolytic viruses have been primarily based on rational design. However, this approach has been met with limited success. An alternative approach employs directed evolution as a means of producing highly selective and potent anticancer viruses. In this method, diverse viruses are grown under conditions that maximize diversity and then passaged under conditions meant to mimic those encountered in the human cancer microenvironment. Viruses which evolve to thrive under this selective pressure are isolated and tested to identify those with increased potency (i.e., ability to replicate and spread) and/or an increased therapeutic window (i.e., differentiated replication and spread on tumor versus normal cells), both of which have potential value but the latter of which defines an oncolytic virus. Using ColoAd1, an oncolytic virus derived by this approach as a prototype, we highlight the benefits of directed evolution, discuss methods to “arm” these novel viruses, and introduce techniques for their genetic modulation and control. |
format | Online Article Text |
id | pubmed-3265225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32652252012-02-06 Oncolytic Viruses: The Power of Directed Evolution Bauzon, Maxine Hermiston, Terry W. Adv Virol Review Article Attempts at developing oncolytic viruses have been primarily based on rational design. However, this approach has been met with limited success. An alternative approach employs directed evolution as a means of producing highly selective and potent anticancer viruses. In this method, diverse viruses are grown under conditions that maximize diversity and then passaged under conditions meant to mimic those encountered in the human cancer microenvironment. Viruses which evolve to thrive under this selective pressure are isolated and tested to identify those with increased potency (i.e., ability to replicate and spread) and/or an increased therapeutic window (i.e., differentiated replication and spread on tumor versus normal cells), both of which have potential value but the latter of which defines an oncolytic virus. Using ColoAd1, an oncolytic virus derived by this approach as a prototype, we highlight the benefits of directed evolution, discuss methods to “arm” these novel viruses, and introduce techniques for their genetic modulation and control. Hindawi Publishing Corporation 2012 2011-07-24 /pmc/articles/PMC3265225/ /pubmed/22312363 http://dx.doi.org/10.1155/2012/586389 Text en Copyright © 2012 M. Bauzon and T. W. Hermiston. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article Bauzon, Maxine Hermiston, Terry W. Oncolytic Viruses: The Power of Directed Evolution |
title | Oncolytic Viruses: The Power of Directed Evolution |
title_full | Oncolytic Viruses: The Power of Directed Evolution |
title_fullStr | Oncolytic Viruses: The Power of Directed Evolution |
title_full_unstemmed | Oncolytic Viruses: The Power of Directed Evolution |
title_short | Oncolytic Viruses: The Power of Directed Evolution |
title_sort | oncolytic viruses: the power of directed evolution |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265225/ https://www.ncbi.nlm.nih.gov/pubmed/22312363 http://dx.doi.org/10.1155/2012/586389 |
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