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Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China

The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7...

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Autores principales: Cui, Li-Jin, Zhang, Chen, Zhang, Ting, Lu, Rou-Jian, Xie, Zheng-De, Zhang, Ling-Lin, Liu, Chuan-Yan, Zhou, Wei-Min, Ruan, Li, Ma, Xue-Jun, Tan, Wen-Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265292/
https://www.ncbi.nlm.nih.gov/pubmed/22315599
http://dx.doi.org/10.1155/2011/129134
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author Cui, Li-Jin
Zhang, Chen
Zhang, Ting
Lu, Rou-Jian
Xie, Zheng-De
Zhang, Ling-Lin
Liu, Chuan-Yan
Zhou, Wei-Min
Ruan, Li
Ma, Xue-Jun
Tan, Wen-Jie
author_facet Cui, Li-Jin
Zhang, Chen
Zhang, Ting
Lu, Rou-Jian
Xie, Zheng-De
Zhang, Ling-Lin
Liu, Chuan-Yan
Zhou, Wei-Min
Ruan, Li
Ma, Xue-Jun
Tan, Wen-Jie
author_sort Cui, Li-Jin
collection PubMed
description The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation.
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spelling pubmed-32652922012-02-07 Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China Cui, Li-Jin Zhang, Chen Zhang, Ting Lu, Rou-Jian Xie, Zheng-De Zhang, Ling-Lin Liu, Chuan-Yan Zhou, Wei-Min Ruan, Li Ma, Xue-Jun Tan, Wen-Jie Adv Virol Research Article The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation. Hindawi Publishing Corporation 2011 2011-07-21 /pmc/articles/PMC3265292/ /pubmed/22315599 http://dx.doi.org/10.1155/2011/129134 Text en Copyright © 2011 Li-Jin Cui et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cui, Li-Jin
Zhang, Chen
Zhang, Ting
Lu, Rou-Jian
Xie, Zheng-De
Zhang, Ling-Lin
Liu, Chuan-Yan
Zhou, Wei-Min
Ruan, Li
Ma, Xue-Jun
Tan, Wen-Jie
Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title_full Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title_fullStr Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title_full_unstemmed Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title_short Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
title_sort human coronaviruses hcov-nl63 and hcov-hku1 in hospitalized children with acute respiratory infections in beijing, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265292/
https://www.ncbi.nlm.nih.gov/pubmed/22315599
http://dx.doi.org/10.1155/2011/129134
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