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Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China
The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265292/ https://www.ncbi.nlm.nih.gov/pubmed/22315599 http://dx.doi.org/10.1155/2011/129134 |
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author | Cui, Li-Jin Zhang, Chen Zhang, Ting Lu, Rou-Jian Xie, Zheng-De Zhang, Ling-Lin Liu, Chuan-Yan Zhou, Wei-Min Ruan, Li Ma, Xue-Jun Tan, Wen-Jie |
author_facet | Cui, Li-Jin Zhang, Chen Zhang, Ting Lu, Rou-Jian Xie, Zheng-De Zhang, Ling-Lin Liu, Chuan-Yan Zhou, Wei-Min Ruan, Li Ma, Xue-Jun Tan, Wen-Jie |
author_sort | Cui, Li-Jin |
collection | PubMed |
description | The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation. |
format | Online Article Text |
id | pubmed-3265292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32652922012-02-07 Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China Cui, Li-Jin Zhang, Chen Zhang, Ting Lu, Rou-Jian Xie, Zheng-De Zhang, Ling-Lin Liu, Chuan-Yan Zhou, Wei-Min Ruan, Li Ma, Xue-Jun Tan, Wen-Jie Adv Virol Research Article The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). We detected HCoV-NL63 and HCoV-HKU1 in specimens collected from May 2008 to March 2010 from patients with ARI aged <7.75 years of age attending the Beijing Children's Hospital. Thirty-two (8.4%) and 57 (14.9%) of 382 specimens tested positive for HCoV-NL63 and HCoV-HKU1, respectively, by real-time RT-PCR. Use of a Luminex xTAG RVP Fast kit showed that coinfection with respiratory syncytial virus and parainfluenza 3 virus was common among patients infected with either virus type. In HCoV-HKU1-infected patients, the predominant clinical symptoms were cough, fever, and expectoration. In HCoV-NL63-infected patients they were cough, fever, and rhinorrhea. Phylogenetic studies showed that the HCoV-HKU1 nucleoprotein gene was relatively conserved compared to NCBI reference sequences, while the 1ab gene of HCoV-NL63 showed more variation. Hindawi Publishing Corporation 2011 2011-07-21 /pmc/articles/PMC3265292/ /pubmed/22315599 http://dx.doi.org/10.1155/2011/129134 Text en Copyright © 2011 Li-Jin Cui et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cui, Li-Jin Zhang, Chen Zhang, Ting Lu, Rou-Jian Xie, Zheng-De Zhang, Ling-Lin Liu, Chuan-Yan Zhou, Wei-Min Ruan, Li Ma, Xue-Jun Tan, Wen-Jie Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title_full | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title_fullStr | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title_full_unstemmed | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title_short | Human Coronaviruses HCoV-NL63 and HCoV-HKU1 in Hospitalized Children with Acute Respiratory Infections in Beijing, China |
title_sort | human coronaviruses hcov-nl63 and hcov-hku1 in hospitalized children with acute respiratory infections in beijing, china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265292/ https://www.ncbi.nlm.nih.gov/pubmed/22315599 http://dx.doi.org/10.1155/2011/129134 |
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