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A novel Gateway(®)-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens
BACKGROUND: Cloning genes into plasmid vectors is one of the key steps for studying gene function. Recently, Invitrogen™ developed a convenient Gateway(® )cloning system based on the site-specific DNA recombination properties of bacteriophage lambda and the cytotoxic protein ccdB, which is lethal to...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265438/ https://www.ncbi.nlm.nih.gov/pubmed/22145613 http://dx.doi.org/10.1186/1746-4811-7-42 |