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Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages
OBJECTIVES: Numerous receptors have been implicated in recognition of pathogenic fungi by macrophages, including the β-glucan receptor dectin-1. The role of scavenger receptors (SRs) in anti-fungal immunity is not well characterized. METHODS: We studied uptake of unopsonized Saccharomycetes cerevisi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SP Birkhäuser Verlag Basel
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265724/ https://www.ncbi.nlm.nih.gov/pubmed/22116297 http://dx.doi.org/10.1007/s00011-011-0395-5 |
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author | Józefowski, Szczepan Yang, Zhiping Marcinkiewicz, Janusz Kobzik, Lester |
author_facet | Józefowski, Szczepan Yang, Zhiping Marcinkiewicz, Janusz Kobzik, Lester |
author_sort | Józefowski, Szczepan |
collection | PubMed |
description | OBJECTIVES: Numerous receptors have been implicated in recognition of pathogenic fungi by macrophages, including the β-glucan receptor dectin-1. The role of scavenger receptors (SRs) in anti-fungal immunity is not well characterized. METHODS: We studied uptake of unopsonized Saccharomycetes cerevisiae (zymosan) and live Candida albicans yeasts as well as zymosan-stimulated H(2)O(2) production in J774 macrophage-like cells and peritoneal exudate macrophages (PEMs). The role of different receptors was assessed with the use of competitive ligands, transfected cells and receptor-deficient macrophages. RESULTS: The uptake of zymosan by untreated J774 cells was mediated approximately half by SRs and half by a β-glucan receptor which was distinct from dectin-1 and not linked to stimulation of H(2)O(2) production. Ligands of β-glucan receptors and of SRs also inhibited uptake of C. albicans by macrophages (J774 cells and PEMs). In macrophages pretreated with a CpG motif-containing oligodeoxynucleotide (CpG-ODN) the relative contribution of SRs to yeast uptake increased and that of β-glucan receptors decreased. Whereas the class A SR MARCO participated in the uptake of both zymosan and C. albicans by CpG-ODN-pretreated, but not untreated macrophages, the related receptor SR-A/CD204 was involved in the uptake of zymosan, but not of C. albicans. The reduction of zymosan-stimulated H(2)O(2) production observed in DS-pretreated J774 cells and in class A SRs-deficient PEMs suggest that class A SRs mediate part of this process. CONCLUSIONS: Our results revealed that SRs belong to a redundant system of receptors for yeasts. Binding of yeasts to different receptors in resting versus CpG-ODN-pre-exposed macrophages may differentially affect polarization of adaptive immune responses. |
format | Online Article Text |
id | pubmed-3265724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SP Birkhäuser Verlag Basel |
record_format | MEDLINE/PubMed |
spelling | pubmed-32657242012-02-03 Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages Józefowski, Szczepan Yang, Zhiping Marcinkiewicz, Janusz Kobzik, Lester Inflamm Res Original Research Paper OBJECTIVES: Numerous receptors have been implicated in recognition of pathogenic fungi by macrophages, including the β-glucan receptor dectin-1. The role of scavenger receptors (SRs) in anti-fungal immunity is not well characterized. METHODS: We studied uptake of unopsonized Saccharomycetes cerevisiae (zymosan) and live Candida albicans yeasts as well as zymosan-stimulated H(2)O(2) production in J774 macrophage-like cells and peritoneal exudate macrophages (PEMs). The role of different receptors was assessed with the use of competitive ligands, transfected cells and receptor-deficient macrophages. RESULTS: The uptake of zymosan by untreated J774 cells was mediated approximately half by SRs and half by a β-glucan receptor which was distinct from dectin-1 and not linked to stimulation of H(2)O(2) production. Ligands of β-glucan receptors and of SRs also inhibited uptake of C. albicans by macrophages (J774 cells and PEMs). In macrophages pretreated with a CpG motif-containing oligodeoxynucleotide (CpG-ODN) the relative contribution of SRs to yeast uptake increased and that of β-glucan receptors decreased. Whereas the class A SR MARCO participated in the uptake of both zymosan and C. albicans by CpG-ODN-pretreated, but not untreated macrophages, the related receptor SR-A/CD204 was involved in the uptake of zymosan, but not of C. albicans. The reduction of zymosan-stimulated H(2)O(2) production observed in DS-pretreated J774 cells and in class A SRs-deficient PEMs suggest that class A SRs mediate part of this process. CONCLUSIONS: Our results revealed that SRs belong to a redundant system of receptors for yeasts. Binding of yeasts to different receptors in resting versus CpG-ODN-pre-exposed macrophages may differentially affect polarization of adaptive immune responses. SP Birkhäuser Verlag Basel 2011-11-25 2012 /pmc/articles/PMC3265724/ /pubmed/22116297 http://dx.doi.org/10.1007/s00011-011-0395-5 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Research Paper Józefowski, Szczepan Yang, Zhiping Marcinkiewicz, Janusz Kobzik, Lester Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title | Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title_full | Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title_fullStr | Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title_full_unstemmed | Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title_short | Scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
title_sort | scavenger receptors and β-glucan receptors participate in the recognition of yeasts by murine macrophages |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265724/ https://www.ncbi.nlm.nih.gov/pubmed/22116297 http://dx.doi.org/10.1007/s00011-011-0395-5 |
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