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Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination
Inner nuclear membrane Sad1/UNC-84 (SUN) proteins interact with outer nuclear membrane (ONM) Klarsicht/ANC-1/Syne homology (KASH) proteins, forming linkers of nucleoskeleton to cytoskeleton conserved from yeast to human and involved in positioning of nuclei and chromosomes. Defects in SUN–KASH bridg...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265956/ https://www.ncbi.nlm.nih.gov/pubmed/22270916 http://dx.doi.org/10.1083/jcb.201108098 |
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author | Zhou, Xiao Graumann, Katja Evans, David E. Meier, Iris |
author_facet | Zhou, Xiao Graumann, Katja Evans, David E. Meier, Iris |
author_sort | Zhou, Xiao |
collection | PubMed |
description | Inner nuclear membrane Sad1/UNC-84 (SUN) proteins interact with outer nuclear membrane (ONM) Klarsicht/ANC-1/Syne homology (KASH) proteins, forming linkers of nucleoskeleton to cytoskeleton conserved from yeast to human and involved in positioning of nuclei and chromosomes. Defects in SUN–KASH bridges are linked to muscular dystrophy, progeria, and cancer. SUN proteins were recently identified in plants, but their ONM KASH partners are unknown. Arabidopsis WPP domain–interacting proteins (AtWIPs) are plant-specific ONM proteins that redundantly anchor Arabidopsis RanGTPase–activating protein 1 (AtRanGAP1) to the nuclear envelope (NE). In this paper, we report that AtWIPs are plant-specific KASH proteins interacting with Arabidopsis SUN proteins (AtSUNs). The interaction is required for both AtWIP1 and AtRanGAP1 NE localization. AtWIPs and AtSUNs are necessary for maintaining the elongated nuclear shape of Arabidopsis epidermal cells. Together, our data identify the first KASH members in the plant kingdom and provide a novel function of SUN–KASH complexes, suggesting that a functionally diverged SUN–KASH bridge is conserved beyond the opisthokonts. |
format | Online Article Text |
id | pubmed-3265956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32659562012-07-23 Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination Zhou, Xiao Graumann, Katja Evans, David E. Meier, Iris J Cell Biol Research Articles Inner nuclear membrane Sad1/UNC-84 (SUN) proteins interact with outer nuclear membrane (ONM) Klarsicht/ANC-1/Syne homology (KASH) proteins, forming linkers of nucleoskeleton to cytoskeleton conserved from yeast to human and involved in positioning of nuclei and chromosomes. Defects in SUN–KASH bridges are linked to muscular dystrophy, progeria, and cancer. SUN proteins were recently identified in plants, but their ONM KASH partners are unknown. Arabidopsis WPP domain–interacting proteins (AtWIPs) are plant-specific ONM proteins that redundantly anchor Arabidopsis RanGTPase–activating protein 1 (AtRanGAP1) to the nuclear envelope (NE). In this paper, we report that AtWIPs are plant-specific KASH proteins interacting with Arabidopsis SUN proteins (AtSUNs). The interaction is required for both AtWIP1 and AtRanGAP1 NE localization. AtWIPs and AtSUNs are necessary for maintaining the elongated nuclear shape of Arabidopsis epidermal cells. Together, our data identify the first KASH members in the plant kingdom and provide a novel function of SUN–KASH complexes, suggesting that a functionally diverged SUN–KASH bridge is conserved beyond the opisthokonts. The Rockefeller University Press 2012-01-23 /pmc/articles/PMC3265956/ /pubmed/22270916 http://dx.doi.org/10.1083/jcb.201108098 Text en © 2012 Zhou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Zhou, Xiao Graumann, Katja Evans, David E. Meier, Iris Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title | Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title_full | Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title_fullStr | Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title_full_unstemmed | Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title_short | Novel plant SUN–KASH bridges are involved in RanGAP anchoring and nuclear shape determination |
title_sort | novel plant sun–kash bridges are involved in rangap anchoring and nuclear shape determination |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265956/ https://www.ncbi.nlm.nih.gov/pubmed/22270916 http://dx.doi.org/10.1083/jcb.201108098 |
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