Cargando…
MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP)
BACKGROUND: The current method to determine the efficacy of chemoprevention in TRAMP mouse model of carcinoma of prostate (CaP) is by extracting and weighing the prostate at different time points or by immunohistochemistry analysis. Non-invasive determination of volumes of prostate glands and semina...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266191/ https://www.ncbi.nlm.nih.gov/pubmed/22165848 http://dx.doi.org/10.1186/1471-2342-11-21 |
_version_ | 1782222142406918144 |
---|---|
author | Arbab, Ali S Shankar, Adarsh Varma, Nadimpalli RS Deeb, Dorrah Gao, Xiaohua Iskander, ASM Janic, Branislava Ali, Meser M Gautam, Subhash C |
author_facet | Arbab, Ali S Shankar, Adarsh Varma, Nadimpalli RS Deeb, Dorrah Gao, Xiaohua Iskander, ASM Janic, Branislava Ali, Meser M Gautam, Subhash C |
author_sort | Arbab, Ali S |
collection | PubMed |
description | BACKGROUND: The current method to determine the efficacy of chemoprevention in TRAMP mouse model of carcinoma of prostate (CaP) is by extracting and weighing the prostate at different time points or by immunohistochemistry analysis. Non-invasive determination of volumes of prostate glands and seminal vesicles before, during and after treatment would be valuable in investigating the efficacy of newer chemopreventive agents in CaP. The purpose of this study was to determine whether in vivo magnetic resonance imaging (MRI) using a 3 tesla clinical MRI system can be used to follow the effect of chemoprevention in TRAMP model of mouse CaP. METHODS: Mice were randomized into control and treated groups. The animals in treated group received 10 µmol/kg of CDDO, 5 days a week for 20 weeks. Animals underwent in vivo MRI of prostate gland and seminal vesicles by a clinical 3 Tesla MRI system just before (at 5 weeks), during and at the end of treatment, at 25 weeks. T1-weighted and fat saturation (FATSAT) multiecho fast spin echo T2- weighted images (T2WI) were acquired. Volume of the prostate glands and seminal vesicles was determined from MR images. T2 signal intensity changes in the seminal vesicles were determined by subtracting higher echo time (TE) from lower TE T2WI. Following treatments all animals were sacrificed, prostate and seminal vesicles collected, and the tissues prepared for histological staining. All data were expressed as mean ± 1 standard deviation. Two-way or multivariate analysis of variance followed by post-hoc test was applied to determine the significant differences. A p-value of <0.05 was considered significant. RESULTS: Histological analysis indicated tumor in 100% of control mice, whereas 10% of the treated mice showed tumor in prostate gland. Both MRI and measured prostate weights showed higher volume/weight in control mouse group. MRI showed significantly higher volume of seminal vesicles in control animals and T2 signal intensity changes in seminal vesicles of control mice indicating higher number of tumor foci, which was also proven by histology. CONCLUSIONS: In vivo MRI is helpful in determining the efficacy of chemoprevention of prostate cancer in TRAMP mice. |
format | Online Article Text |
id | pubmed-3266191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32661912012-01-26 MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) Arbab, Ali S Shankar, Adarsh Varma, Nadimpalli RS Deeb, Dorrah Gao, Xiaohua Iskander, ASM Janic, Branislava Ali, Meser M Gautam, Subhash C BMC Med Imaging Research Article BACKGROUND: The current method to determine the efficacy of chemoprevention in TRAMP mouse model of carcinoma of prostate (CaP) is by extracting and weighing the prostate at different time points or by immunohistochemistry analysis. Non-invasive determination of volumes of prostate glands and seminal vesicles before, during and after treatment would be valuable in investigating the efficacy of newer chemopreventive agents in CaP. The purpose of this study was to determine whether in vivo magnetic resonance imaging (MRI) using a 3 tesla clinical MRI system can be used to follow the effect of chemoprevention in TRAMP model of mouse CaP. METHODS: Mice were randomized into control and treated groups. The animals in treated group received 10 µmol/kg of CDDO, 5 days a week for 20 weeks. Animals underwent in vivo MRI of prostate gland and seminal vesicles by a clinical 3 Tesla MRI system just before (at 5 weeks), during and at the end of treatment, at 25 weeks. T1-weighted and fat saturation (FATSAT) multiecho fast spin echo T2- weighted images (T2WI) were acquired. Volume of the prostate glands and seminal vesicles was determined from MR images. T2 signal intensity changes in the seminal vesicles were determined by subtracting higher echo time (TE) from lower TE T2WI. Following treatments all animals were sacrificed, prostate and seminal vesicles collected, and the tissues prepared for histological staining. All data were expressed as mean ± 1 standard deviation. Two-way or multivariate analysis of variance followed by post-hoc test was applied to determine the significant differences. A p-value of <0.05 was considered significant. RESULTS: Histological analysis indicated tumor in 100% of control mice, whereas 10% of the treated mice showed tumor in prostate gland. Both MRI and measured prostate weights showed higher volume/weight in control mouse group. MRI showed significantly higher volume of seminal vesicles in control animals and T2 signal intensity changes in seminal vesicles of control mice indicating higher number of tumor foci, which was also proven by histology. CONCLUSIONS: In vivo MRI is helpful in determining the efficacy of chemoprevention of prostate cancer in TRAMP mice. BioMed Central 2011-12-13 /pmc/articles/PMC3266191/ /pubmed/22165848 http://dx.doi.org/10.1186/1471-2342-11-21 Text en Copyright ©2011 Arbab et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Arbab, Ali S Shankar, Adarsh Varma, Nadimpalli RS Deeb, Dorrah Gao, Xiaohua Iskander, ASM Janic, Branislava Ali, Meser M Gautam, Subhash C MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title | MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title_full | MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title_fullStr | MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title_full_unstemmed | MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title_short | MRI to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (TRAMP) |
title_sort | mri to assess chemoprevention in transgenic adenocarcinoma of mouse prostate (tramp) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266191/ https://www.ncbi.nlm.nih.gov/pubmed/22165848 http://dx.doi.org/10.1186/1471-2342-11-21 |
work_keys_str_mv | AT arbabalis mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT shankaradarsh mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT varmanadimpallirs mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT deebdorrah mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT gaoxiaohua mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT iskanderasm mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT janicbranislava mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT alimeserm mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp AT gautamsubhashc mritoassesschemopreventionintransgenicadenocarcinomaofmouseprostatetramp |