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Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice
Both acute and chronic apelin treatment have been shown to improve insulin sensitivity in mice. However, the effects of apelin on fatty acid oxidation (FAO) during obesity-related insulin resistance have not yet been addressed. Thus, the aim of the current study was to determine the impact of chroni...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Diabetes Association
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266414/ https://www.ncbi.nlm.nih.gov/pubmed/22210322 http://dx.doi.org/10.2337/db11-0100 |
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author | Attané, Camille Foussal, Camille Le Gonidec, Sophie Benani, Alexandre Daviaud, Danièle Wanecq, Estelle Guzmán-Ruiz, Rocío Dray, Cédric Bezaire, Veronic Rancoule, Chloé Kuba, Keiji Ruiz-Gayo, Mariano Levade, Thierry Penninger, Josef Burcelin, Rémy Pénicaud, Luc Valet, Philippe Castan-Laurell, Isabelle |
author_facet | Attané, Camille Foussal, Camille Le Gonidec, Sophie Benani, Alexandre Daviaud, Danièle Wanecq, Estelle Guzmán-Ruiz, Rocío Dray, Cédric Bezaire, Veronic Rancoule, Chloé Kuba, Keiji Ruiz-Gayo, Mariano Levade, Thierry Penninger, Josef Burcelin, Rémy Pénicaud, Luc Valet, Philippe Castan-Laurell, Isabelle |
author_sort | Attané, Camille |
collection | PubMed |
description | Both acute and chronic apelin treatment have been shown to improve insulin sensitivity in mice. However, the effects of apelin on fatty acid oxidation (FAO) during obesity-related insulin resistance have not yet been addressed. Thus, the aim of the current study was to determine the impact of chronic treatment on lipid use, especially in skeletal muscles. High-fat diet (HFD)-induced obese and insulin-resistant mice treated by an apelin injection (0.1 μmol/kg/day i.p.) during 4 weeks had decreased fat mass, glycemia, and plasma levels of triglycerides and were protected from hyperinsulinemia compared with HFD PBS-treated mice. Indirect calorimetry experiments showed that apelin-treated mice had a better use of lipids. The complete FAO, the oxidative capacity, and mitochondrial biogenesis were increased in soleus of apelin-treated mice. The action of apelin was AMP-activated protein kinase (AMPK) dependent since all the effects studied were abrogated in HFD apelin-treated mice with muscle-specific inactive AMPK. Finally, the apelin-stimulated improvement of oxidative capacity led to decreased levels of acylcarnitines and enhanced insulin-stimulated glucose uptake in soleus. Thus, by promoting complete lipid use in muscle of insulin-resistant mice through mitochondrial biogenesis and tighter matching between FAO and the tricarboxylic acid cycle, apelin treatment could contribute to insulin sensitivity improvement. |
format | Online Article Text |
id | pubmed-3266414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-32664142013-02-01 Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice Attané, Camille Foussal, Camille Le Gonidec, Sophie Benani, Alexandre Daviaud, Danièle Wanecq, Estelle Guzmán-Ruiz, Rocío Dray, Cédric Bezaire, Veronic Rancoule, Chloé Kuba, Keiji Ruiz-Gayo, Mariano Levade, Thierry Penninger, Josef Burcelin, Rémy Pénicaud, Luc Valet, Philippe Castan-Laurell, Isabelle Diabetes Metabolism Both acute and chronic apelin treatment have been shown to improve insulin sensitivity in mice. However, the effects of apelin on fatty acid oxidation (FAO) during obesity-related insulin resistance have not yet been addressed. Thus, the aim of the current study was to determine the impact of chronic treatment on lipid use, especially in skeletal muscles. High-fat diet (HFD)-induced obese and insulin-resistant mice treated by an apelin injection (0.1 μmol/kg/day i.p.) during 4 weeks had decreased fat mass, glycemia, and plasma levels of triglycerides and were protected from hyperinsulinemia compared with HFD PBS-treated mice. Indirect calorimetry experiments showed that apelin-treated mice had a better use of lipids. The complete FAO, the oxidative capacity, and mitochondrial biogenesis were increased in soleus of apelin-treated mice. The action of apelin was AMP-activated protein kinase (AMPK) dependent since all the effects studied were abrogated in HFD apelin-treated mice with muscle-specific inactive AMPK. Finally, the apelin-stimulated improvement of oxidative capacity led to decreased levels of acylcarnitines and enhanced insulin-stimulated glucose uptake in soleus. Thus, by promoting complete lipid use in muscle of insulin-resistant mice through mitochondrial biogenesis and tighter matching between FAO and the tricarboxylic acid cycle, apelin treatment could contribute to insulin sensitivity improvement. American Diabetes Association 2012-02 2012-01-17 /pmc/articles/PMC3266414/ /pubmed/22210322 http://dx.doi.org/10.2337/db11-0100 Text en © 2012 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details. |
spellingShingle | Metabolism Attané, Camille Foussal, Camille Le Gonidec, Sophie Benani, Alexandre Daviaud, Danièle Wanecq, Estelle Guzmán-Ruiz, Rocío Dray, Cédric Bezaire, Veronic Rancoule, Chloé Kuba, Keiji Ruiz-Gayo, Mariano Levade, Thierry Penninger, Josef Burcelin, Rémy Pénicaud, Luc Valet, Philippe Castan-Laurell, Isabelle Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title | Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title_full | Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title_fullStr | Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title_full_unstemmed | Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title_short | Apelin Treatment Increases Complete Fatty Acid Oxidation, Mitochondrial Oxidative Capacity, and Biogenesis in Muscle of Insulin-Resistant Mice |
title_sort | apelin treatment increases complete fatty acid oxidation, mitochondrial oxidative capacity, and biogenesis in muscle of insulin-resistant mice |
topic | Metabolism |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266414/ https://www.ncbi.nlm.nih.gov/pubmed/22210322 http://dx.doi.org/10.2337/db11-0100 |
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