An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants

In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD i...

Descripción completa

Detalles Bibliográficos
Autores principales: Hogg, Bridget V, Kacprzyk, Joanna, Molony, Elizabeth M, O'Reilly, Conor, Gallagher, Thomas F, Gallois, Patrick, McCabe, Paul F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266644/
https://www.ncbi.nlm.nih.gov/pubmed/22165954
http://dx.doi.org/10.1186/1746-4811-7-45
_version_ 1782222202329890816
author Hogg, Bridget V
Kacprzyk, Joanna
Molony, Elizabeth M
O'Reilly, Conor
Gallagher, Thomas F
Gallois, Patrick
McCabe, Paul F
author_facet Hogg, Bridget V
Kacprzyk, Joanna
Molony, Elizabeth M
O'Reilly, Conor
Gallagher, Thomas F
Gallois, Patrick
McCabe, Paul F
author_sort Hogg, Bridget V
collection PubMed
description In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.
format Online
Article
Text
id pubmed-3266644
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32666442012-01-27 An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants Hogg, Bridget V Kacprzyk, Joanna Molony, Elizabeth M O'Reilly, Conor Gallagher, Thomas F Gallois, Patrick McCabe, Paul F Plant Methods Methodology In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation. BioMed Central 2011-12-13 /pmc/articles/PMC3266644/ /pubmed/22165954 http://dx.doi.org/10.1186/1746-4811-7-45 Text en Copyright ©2011 Hogg et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Hogg, Bridget V
Kacprzyk, Joanna
Molony, Elizabeth M
O'Reilly, Conor
Gallagher, Thomas F
Gallois, Patrick
McCabe, Paul F
An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title_full An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title_fullStr An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title_full_unstemmed An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title_short An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
title_sort in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266644/
https://www.ncbi.nlm.nih.gov/pubmed/22165954
http://dx.doi.org/10.1186/1746-4811-7-45
work_keys_str_mv AT hoggbridgetv aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT kacprzykjoanna aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT molonyelizabethm aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT oreillyconor aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT gallagherthomasf aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT galloispatrick aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT mccabepaulf aninvivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT hoggbridgetv invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT kacprzykjoanna invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT molonyelizabethm invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT oreillyconor invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT gallagherthomasf invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT galloispatrick invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants
AT mccabepaulf invivoroothairassayfordeterminingratesofapoptoticlikeprogrammedcelldeathinplants

Ejemplares similares