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Development of RNA Aptamer and its Ligand Binding Assay on Microchip Electrophoresis

Microchip electrophoresis (ME) coupled with fluorescence detection was used to estimate the binding activity of aptamer in each systematic evolution of ligands by exponential enrichment (SELEX) round for a target molecule. This approach is a non-radioisotopic, rapid and simple platform, and electrop...

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Detalles Bibliográficos
Autores principales: Ohno, Ken-ichi, Nakata, Chikara, Sano, Yoshihiro, Nishikawa, Fumiko, Nishikawa, Satoshi, Arakawa, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267091/
https://www.ncbi.nlm.nih.gov/pubmed/22291866
http://dx.doi.org/10.2174/1875397301206010001