Cargando…

Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor

A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotub...

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Ahmed, Amir, Ndangili, Peter M., Jahed, Nazeem, Baker, Priscilla G. L., Iwuoha, Emmanuel I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267205/
https://www.ncbi.nlm.nih.gov/pubmed/22303157
http://dx.doi.org/10.3390/s91209965
_version_ 1782222259883081728
author Al-Ahmed, Amir
Ndangili, Peter M.
Jahed, Nazeem
Baker, Priscilla G. L.
Iwuoha, Emmanuel I.
author_facet Al-Ahmed, Amir
Ndangili, Peter M.
Jahed, Nazeem
Baker, Priscilla G. L.
Iwuoha, Emmanuel I.
author_sort Al-Ahmed, Amir
collection PubMed
description A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (∼90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)(-1) and a detection limit of 0.185 μM for H(2)O(2). Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C.
format Online
Article
Text
id pubmed-3267205
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-32672052012-02-02 Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor Al-Ahmed, Amir Ndangili, Peter M. Jahed, Nazeem Baker, Priscilla G. L. Iwuoha, Emmanuel I. Sensors (Basel) Article A novel enzyme immobilization platform was prepared on a platinum disk working electrode by polymerizing aniline inside the interstitial pores of polyester sulphonic acid sodium salt (PESA). Scanning electron microscopy study showed the formation of homogeneous sulphonated polyaniline (PANI) nanotubes (∼90 nm) and thermogravimetric analysis (TGA) confirmed that the nanotubes were stable up to 230 °C. The PANI:PESA nanocomposite showed a quasi-reversible redox behaviour in phosphate buffer saline. Horseradish peroxidase (HRP) was immobilized on to this modified electrode for hydrogen peroxide detection. The biosensor gave a sensitivity of 1.33 μA (μM)(-1) and a detection limit of 0.185 μM for H(2)O(2). Stability experiments showed that the biosensor retained more than 64% of its initial sensitivity over four days of storage at 4 °C. Molecular Diversity Preservation International (MDPI) 2009-12-08 /pmc/articles/PMC3267205/ /pubmed/22303157 http://dx.doi.org/10.3390/s91209965 Text en © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Al-Ahmed, Amir
Ndangili, Peter M.
Jahed, Nazeem
Baker, Priscilla G. L.
Iwuoha, Emmanuel I.
Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title_full Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title_fullStr Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title_full_unstemmed Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title_short Polyester Sulphonic Acid Interstitial Nanocomposite Platform for Peroxide Biosensor
title_sort polyester sulphonic acid interstitial nanocomposite platform for peroxide biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267205/
https://www.ncbi.nlm.nih.gov/pubmed/22303157
http://dx.doi.org/10.3390/s91209965
work_keys_str_mv AT alahmedamir polyestersulphonicacidinterstitialnanocompositeplatformforperoxidebiosensor
AT ndangilipeterm polyestersulphonicacidinterstitialnanocompositeplatformforperoxidebiosensor
AT jahednazeem polyestersulphonicacidinterstitialnanocompositeplatformforperoxidebiosensor
AT bakerpriscillagl polyestersulphonicacidinterstitialnanocompositeplatformforperoxidebiosensor
AT iwuohaemmanueli polyestersulphonicacidinterstitialnanocompositeplatformforperoxidebiosensor