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Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)

BACKGROUND: The transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. RESULTS: The transcriptomes of WT a...

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Autores principales: Yu, Keqin, Xu, Qiang, Da, Xinlei, Guo, Fei, Ding, Yuduan, Deng, Xiuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267696/
https://www.ncbi.nlm.nih.gov/pubmed/22230690
http://dx.doi.org/10.1186/1471-2164-13-10
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author Yu, Keqin
Xu, Qiang
Da, Xinlei
Guo, Fei
Ding, Yuduan
Deng, Xiuxin
author_facet Yu, Keqin
Xu, Qiang
Da, Xinlei
Guo, Fei
Ding, Yuduan
Deng, Xiuxin
author_sort Yu, Keqin
collection PubMed
description BACKGROUND: The transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. RESULTS: The transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening. CONCLUSION: A description of the transcriptomic changes occurring during fruit development and ripening was obtained in sweet orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant.
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spelling pubmed-32676962012-01-28 Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis) Yu, Keqin Xu, Qiang Da, Xinlei Guo, Fei Ding, Yuduan Deng, Xiuxin BMC Genomics Research Article BACKGROUND: The transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. RESULTS: The transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening. CONCLUSION: A description of the transcriptomic changes occurring during fruit development and ripening was obtained in sweet orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant. BioMed Central 2012-01-10 /pmc/articles/PMC3267696/ /pubmed/22230690 http://dx.doi.org/10.1186/1471-2164-13-10 Text en Copyright ©2012 Yu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Keqin
Xu, Qiang
Da, Xinlei
Guo, Fei
Ding, Yuduan
Deng, Xiuxin
Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title_full Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title_fullStr Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title_full_unstemmed Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title_short Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)
title_sort transcriptome changes during fruit development and ripening of sweet orange (citrus sinensis)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267696/
https://www.ncbi.nlm.nih.gov/pubmed/22230690
http://dx.doi.org/10.1186/1471-2164-13-10
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